In Vivo Detection of Intervertebral Disk Injury Using a Radiolabeled Monoclonal Antibody Against Keratan Sulfate

Journal of Nuclear Medicine

Eeva Kääpä¹  Kalevi J.A. Kairemo¹  Outi M. Laitinen¹  Timo Hyytinen¹  Sirkka-Liisa Karonen¹  Anu K. Lappalainen¹  Mats Grönblad¹ 

[1] Departments of Clinical Chemistry, Physical Medicine and Rehabilitation, and Thoracic and Cardiovascular Surgery, Helsinki University Central Hospital, Helsinki; and Faculty of Veterinary Medicine, Department of Clinical Veterinary Sciences, University of Helsinki, Helsinki, Finland Departments of Clinical Chemistry, Physical Medicine and Rehabilitation, and Thoracic and Cardiovascular Surgery, Helsinki University Central Hospital, Helsinki; and Faculty of Veterinary Medicine, Department of Clinical Veterinary Sciences, University of Helsinki, Helsinki, Finland Departments of Clinical Chemistry, Physical Medicine and Rehabilitation, and Thoracic and Cardiovascular Surgery, Helsinki University Central Hospital, Helsinki; and Faculty of Veterinary Medicine, Department of Clinical Veterinary Sciences, University of Helsinki, Helsinki, Finland

关键词: monoclonal antibodies;    keratan sulfate;    disk injury;    intervertebral disk;    cartilaginous tissue;    radioimmunodetection;   

DOI  :  

学科分类：医学（综合）

来源: Society of Nuclear Medicine

【 摘 要 】

In the intervertebral disk, proteoglycans form the major part of the extracellular matrix, surrounding chondrocytelike disk cells. Keratan sulfate is a major constituent of proteoglycans. Methods: We have radioiodinated a monoclonal antibody raised against keratan sulfate. This antibody was injected into rats (n = 6), and the biodistribution was studied. A model of intervertebral disk injury was developed, and two tail disks in each animal with both acute (2 wk old) and subacute (7 wk old) injuries were studied for in vivo antibody uptake. Results: The biodistribution at 72 h was as follows: blood, 0.0018 percentage injected dose per gram of tissue (%ID/g); lung, 0.0106 %ID/g; esophagus, 0.0078 %ID/g; kidney, 0.0063 %ID/g; liver, 0.0047 %ID/g; spleen, 0.0046 %ID/g; heart, 0.0036 %ID/g; thyroid, 0.0034 %ID/g; muscle, 0.0017 %ID/g; and bone, 0.0016 %ID/g. In the subacute stage, a significant difference (P < 0.006) was found in antibody uptake between injured disks (n = 12) and adjacent healthy disks (n = 12). In vivo γ imaging showed increased uptake in other animals having lumbar disk injuries (2, 7, and 17 d after injury). Cartilage tissue, such as the trachea, was studied separately and showed extremely high antibody uptake, 0.10 %ID/g. Rat trachea was also visualized on γ images. Conclusion: Our data suggest that antibodies against nucleus pulposus components, such as proteoglycans, can be used for in vivo detection of intervertebral disk injury. This finding is in spite of the minimal circulation present in intervertebral disks.

【 授权许可】

Unknown