Imaging Cyclophosphamide-Induced Intramedullary Apoptosis in Rats Using 99mTc-Radiolabeled Annexin V

Journal of Nuclear Medicine

Johnathan F. Tait¹  Louie Naumovski¹  Anneke M. Post¹  Francis G. Blankenberg¹  H. William Strauss¹ 

[1] Divisions of Pediatric Radiology and Pediatric Hematology/Oncology, Lucile Salter Packard Children’s Hospital, Stanford; Division of Nuclear Medicine, Department of Radiology, Stanford, California; and Department of Laboratory Medicine, University of Washington, Seattle, Washington Divisions of Pediatric Radiology and Pediatric Hematology/Oncology, Lucile Salter Packard Children’s Hospital, Stanford; Division of Nuclear Medicine, Department of Radiology, Stanford, California; and Department of Laboratory Medicine, University of Washington, Seattle, Washington Divisions of Pediatric Radiology and Pediatric Hematology/Oncology, Lucile Salter Packard Children’s Hospital, Stanford; Division of Nuclear Medicine, Department of Radiology, Stanford, California; and Department of Laboratory Medicine, University of Washington, Seattle, Washington

关键词: annexin V;    bone marrow;    apoptosis;    radionuclide;   

DOI  :  

学科分类：医学（综合）

来源: Society of Nuclear Medicine

【 摘 要 】

Intramedullary apoptosis of hematopoietic tissue is believed to play a major role in the pathophysiology of myelodysplastic syndrome. Annexin V, a specific marker of the early to intermediate phases of apoptosis, has been applied to the in vitro study of bone marrow aspirates. A noninvasive measure of intramedullary apoptosis in vivo that could serially monitor the clinical progression of myelodysplastic syndrome may be helpful. Methods: We used 99mTc-radiolabeled annexin V and radionuclide gamma camera imaging to serially study the sites, extent, and severity of intramedullary apoptosis induced by cyclophosphamide treatment. Results: Intravenously administered radiolabeled annexin V localized preferentially in the femur, pelvis, vertebrae, and spleen; increased uptake in these organs was easily visualized as early as 8 h after injection of 100 mg/kg cyclophosphamide in 8- to 10-wk-old animals. Higher doses of cyclophosphamide (150 mg/kg) in animals of the same age increased annexin V uptake in the bone marrow and splenic tissue and delayed recovery of these organs as seen histologically compared with lower doses. Older animals, 5–6 mo old, showed a slower response to cyclophosphamide treatment and delayed recovery of bone marrow and splenic tissues. Conclusion: Radiolabeled annexin V can be used to detect and directly quantify the degree of intramedullary and splenic apoptosis in a noninvasive fashion using current clinical radionuclide imaging equipment. Annexin V imaging may be useful clinically in the diagnosis and management of myelodysplastic syndrome.

【 授权许可】

Unknown