Rapid determination of a fluorinated gas in a lipid microbubble contrast agent by ultrasound-mediated microbubble destruction and GC-MS

Analytical Methods

Diyi Pan²  Lei Li²  Xiao Li¹  Li Yang²  Zhiliang Chen²  Ying Wang³  Jing Peng²  Lianbing Hou² 

[1] Department of Pharmacy, The Affiliated Hospital of Qingdao University,Qingdao,ChinaDepartment of Pharmacy, The Affiliated Hospital of Qingdao University,Qingdao,ChinaDepartment of Pharmacy, The Affiliated Hospital of Qingdao University,Qingdao,China;Department of Pharmacy, Nanfang Hospital, Southern Medical University,Guangzhou 510515,ChinaDepartment of Pharmacy, Nanfang Hospital, Southern Medical University,Guangzhou 510515,ChinaDepartment of Pharmacy, Nanfang Hospital, Southern Medical University,Guangzhou 510515,China;Institute of Cancer Research, Southern Medical University,Guangzhou 510515,ChinaInstitute of Cancer Research, Southern Medical University,Guangzhou 510515,ChinaInstitute of Cancer Research, Southern Medical University,Guangzhou 510515,China

DOI  :  10.1039/C5AY01912E

学科分类：分析化学

来源: Royal Society of Chemistry

【 摘 要 】

A rapid and efficient method was developed for the determination of a fluorinated gas in a lipid microbubble contrast agent, by ultrasound-mediated microbubble destruction (UMD) and gas chromatography-mass spectrometry (GC-MS). UMD was performed using bath sonication. The microbubble destruction was assessed according to the change in the microbubble size, measured by dynamic light scattering (DLS). The results indicated that 20 min bath sonication at 40 kHz was sufficient for disruption of 25 μL of a microbubble agent [octafluoropropane (OFP)-containing lipid microbubbles] in 1 mL saline. The released OFP was then determined by GC-MS with electron impact ionization in the selected-ion-monitoring (SIM) mode using external standardization. A limit of quantification of 9.22 ng OFP and a linearity range of 30.74 to 30742.24 ng were obtained. Validation of the method showed satisfactory specificity, precision and accuracy. Its validity was further supported by its application in the analysis of real samples with determined values consistent with those by the US Pharmacopeia method. In particular, the sample preparation time was shortened from 3 h to 20 min. In this study, an alternative sample preparation method has been established for microbubble gas content determination, which is rapid and as accurate as classic methods.

【 授权许可】

Unknown