Stable gold nanoparticles as a novel peroxidase mimic for colorimetric detection of cysteine

Analytical Methods

Rong Sheng Li¹  Hai Liu¹  Jian Wang²  Hong Zhi Zhang¹  Bin Bin Chen³  Cheng Zhi Huang⁴ 

[1] Key Laboratory on Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University,Chongqing 400715,ChinaKey Laboratory on Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University,Chongqing 400715,ChinaKey Laboratory on Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University,Chongqing 400715,China;Key Laboratory on Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University,Chongqing 400715,ChinaState Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University,Changsha 410082,ChinaKey Laboratory on Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University,Chongqing 400715,ChinaKey Laboratory on Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University,Chongqing 400715,ChinaState Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University,Changsha 410082,ChinaState Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University,Changsha 410082,ChinaKey Laboratory on Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University,Chongqing 400715,ChinaState Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University,Changsha 410082,China;College of Chemistry and Chemical Engineering, Southwest University,Chongqing 400715,ChinaCollege of Chemistry and Chemical Engineering, Southwest University,Chongqing 400715,ChinaCollege of Chemistry and Chemical Engineering, Southwest University,Chongqing 400715,China;Key Laboratory on Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University,Chongqing 400715,ChinaCollege of Chemistry and Chemical Engineering, Southwest University,Chongqing 400715,ChinaKey Laboratory on Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University,Chongqing 400715,ChinaKey Laboratory on Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University,Chongqing 400715,ChinaCollege of Chemistry and Chemical Engineering, Southwest University,Chongqing 400715,ChinaCollege of Chemistry and Chemical Engineering, Southwest University,Chongqing 400715,ChinaKey Laboratory on Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University,Chongqing 400715,ChinaCollege of Chemistry and Chemical Engineering, Southwest University,Chongqing 400715,China

DOI  :  10.1039/C6AY00367B

学科分类：分析化学

来源: Royal Society of Chemistry

【 摘 要 】

In this work, a novel method for colorimetric detection of cysteine was proposed based on the inhibition of peroxidase-like behavior of AuNPs. Kiwi juice prepared gold nanoparticles presented strong catalytic activity, which could catalyze H2O2–TMB to generate a blue product, resulting in strong absorption at 650 nm. In HAc–NaAc (pH 4.0) buffer, cysteine induced the aggregation of gold nanoparticles through covalent Au–S bonds and electrostatic as well as hydrogen bonding, leading to the reduction of the catalytic activity of gold nanoparticles with a weakened blue color product and a lower absorbance. Under optimal conditions, the concentrations of cysteine were proportional to the degree of reduced absorbance. This approach offers a new, simple, sensitive and selective assay for cysteine.

【 授权许可】

Unknown