Determination of the active ingredients in simple-recipe ointments by centrifugal partition chromatography

Analytical Methods

Bo-hai Jiang¹  Lei Wang¹  Yue-qing Li¹  Wei-jie Zhao¹ 

[1] School of Pharmaceutical Science and Technology, State Key Laboratory of Fine Chemicals, Dalian University of Technology,Dalian 116024,P.R. ChinaSchool of Pharmaceutical Science and Technology, State Key Laboratory of Fine Chemicals, Dalian University of Technology,Dalian 116024,P.R. ChinaSchool of Pharmaceutical Science and Technology, State Key Laboratory of Fine Chemicals, Dalian University of Technology,Dalian 116024,P.R. China

DOI  :  10.1039/C4AY00131A

学科分类：分析化学

来源: Royal Society of Chemistry

【 摘 要 】

The objective of this study was to establish a centrifugal partition chromatography (CPC) method for determination of the active ingredients in single-recipe ointments. The mechanism of this method was that the active ingredients were investigated using a UV detector after being extracted from the ointments by CPC, which worked as a fine separating funnel. Three ointments, containing salicylic acid, clotrimazole or aciclovir, were investigated using this method. The calibration curves of all three of the preparations were linear with a R2 value higher than 0.998 in the tested concentration range, and the precision RSDs for salicylic acid, clotrimazole and aciclovir were 1.3%, 0.9% and 1.5%, respectively. Compared with the HPLC method, the CPC method had equivalent precision and accuracy, but could directly use the ointment solution as a sample instead of needing a complex sample preparation process. Furthermore, the CPC method also had several advantages in economic cost. In summary, the CPC method is a potential optimized technique to determine ointment formulations.

【 授权许可】

Unknown