Separation, identification and fast determination of organophosphate pesticide methidathion in tea leaves by thin layer chromatography–surface-enhanced Raman scattering

Analytical Methods

Zhiyou Zhang¹  Zhengjun Gong⁴  Chaoping Yao⁶  Yonghong Wang⁵  Cong Wang³  Meikun Fan⁴  Xiaowei Guo²  Fansheng Cheng³ 

[1] Institute of Nanophotonics Technology, School of Physical Science and Technology, Sichuan University,Chengdu,ChinaInstitute of Nanophotonics Technology, School of Physical Science and Technology, Sichuan University,Chengdu,ChinaInstitute of Nanophotonics Technology, School of Physical Science and Technology, Sichuan University,Chengdu,China;Department of Optoelectronic Information, University of Electronic Science and Technology of China,Chengdu,ChinaDepartment of Optoelectronic Information, University of Electronic Science and Technology of China,Chengdu,ChinaDepartment of Optoelectronic Information, University of Electronic Science and Technology of China,Chengdu,China;Chengdu Green Energy and Green Manufacturing R&D Centre,Chengdu,ChinaChengdu Green Energy and Green Manufacturing R&D Centre,Chengdu,ChinaChengdu Green Energy and Green Manufacturing R&D Centre,Chengdu,China;Faculty of Geosciences and Environmental Engineering, Southwest Jiaotong University,Chengdu,ChinaFaculty of Geosciences and Environmental Engineering, Southwest Jiaotong University,Chengdu,ChinaFaculty of Geosciences and Environmental Engineering, Southwest Jiaotong University,Chengdu,China;Center for Animal Disease Control and Prevention of Chongqing,Chongqing,ChinaCenter for Animal Disease Control and Prevention of Chongqing,Chongqing,ChinaCenter for Animal Disease Control and Prevention of Chongqing,Chongqing,China;Department of Optoelectronic Information, University of Electronic Science and Technology of China,Chengdu,ChinaChengdu Green Energy and Green Manufacturing R&D Centre,Chengdu,ChinaDepartment of Optoelectronic Information, University of Electronic Science and Technology of China,Chengdu,ChinaDepartment of Optoelectronic Information, University of Electronic Science and Technology of China,Chengdu,ChinaChengdu Green Energy and Green Manufacturing R&D Centre,Chengdu,ChinaChengdu Green Energy and Green Manufacturing R&D Centre,Chengdu,ChinaDepartment of Optoelectronic Information, University of Electronic Science and Technology of China,Chengdu,ChinaChengdu Green Energy and Green Manufacturing R&D Centre,Chengdu,China

DOI  :  10.1039/C3AY41152D

学科分类：分析化学

来源: Royal Society of Chemistry

【 摘 要 】

In this work, a fast, sensitive and convenient method for the on-site separation, identification and determination of organophosphate pesticide methidathion in tea leaves was reported, by the use of thin layer chromatography (TLC) in combination with surface-enhanced Raman spectroscopy (SERS). Five different organophosphate pesticides have been successfully separated and identified. Factors that affect the SERS detection sensitivity of methidathion, such as the brand of TLC plate, material and concentration of metallic nanoparticles (NPs), have been examined. It is found that the limit of quantification for methidathion is 0.1 ppm. Spiked tea samples of different kinds and brands containing methidathion at the ppm level have been tested, with recovery rates in the range of 86–113%. The proposed method for the fast on-site determination of pesticide may help to address food safety concerns in the general public.

【 授权许可】

Unknown