Integrated glycomic analysis of ovarian cancer side population cells

Clinical Proteomics

Ran Zhao⁴  Yisheng Wang¹  Xiaoxia Liu¹  Caiting Yang⁵  Lili Li⁵  Peike Peng⁵  Ruihuan Qin⁵  Hao Wu⁵  Yuanyuan Ruan⁵  Shifang Ren⁵  Xingwang Zhang⁵  Congjian Xu⁶  Xiaoxiang Jie³  Haiyan Tai²  Jianxin Gu⁵  Wenjun Qin⁵  Miaomiao Shao⁵  Mengyu Zhang² 

[1] Obstetrics and Gynecology Hospital of Fudan University, Shanghai, People’s Republic of ChinaShanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, People’s Republic of ChinaObstetrics and Gynecology Hospital of Fudan University, Shanghai, People’s Republic of ChinaObstetrics and Gynecology Hospital of Fudan University, Shanghai, People’s Republic of ChinaShanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, People’s Republic of ChinaShanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, People’s Republic of ChinaObstetrics and Gynecology Hospital of Fudan University, Shanghai, People’s Republic of ChinaShanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, People’s Republic of China;Obstetrics and Gynecology Hospital of Fudan University, Shanghai, People’s Republic of ChinaObstetrics and Gynecology Hospital of Fudan University, Shanghai, People’s Republic of ChinaObstetrics and Gynecology Hospital of Fudan University, Shanghai, People’s Republic of China;Department of Obstetrics and Gynecology of Shanghai Medical School, Fudan University, Shanghai, People’s Republic of ChinaDepartment of Obstetrics and Gynecology of Shanghai Medical School, Fudan University, Shanghai, People’s Republic of ChinaDepartment of Obstetrics and Gynecology of Shanghai Medical School, Fudan University, Shanghai, People’s Republic of China;Institute of Biomedical Sciences, Fudan University, Shanghai, People’s Republic of ChinaInstitute of Biomedical Sciences, Fudan University, Shanghai, People’s Republic of ChinaInstitute of Biomedical Sciences, Fudan University, Shanghai, People’s Republic of China;Key Laboratory of Glycoconjugate Research Ministry of Public Health, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, People’s Republic of ChinaKey Laboratory of Glycoconjugate Research Ministry of Public Health, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, People’s Republic of ChinaKey Laboratory of Glycoconjugate Research Ministry of Public Health, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, People’s Republic of China;Institute of Biomedical Sciences, Fudan University, Shanghai, People’s Republic of ChinaObstetrics and Gynecology Hospital of Fudan University, Shanghai, People’s Republic of ChinaDepartment of Obstetrics and Gynecology of Shanghai Medical School, Fudan University, Shanghai, People’s Republic of ChinaShanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, People’s Republic of ChinaInstitute of Biomedical Sciences, Fudan University, Shanghai, People’s Republic of ChinaInstitute of Biomedical Sciences, Fudan University, Shanghai, People’s Republic of ChinaObstetrics and Gynecology Hospital of Fudan University, Shanghai, People’s Republic of ChinaDepartment of Obstetrics and Gynecology of Shanghai Medical School, Fudan University, Shanghai, People’s Republic of ChinaShanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, People’s Republic of ChinaObstetrics and Gynecology Hospital of Fudan University, Shanghai, People’s Republic of ChinaInstitute of Biomedical Sciences, Fudan University, Shanghai, People’s Republic of ChinaObstetrics and Gynecology Hospital of Fudan University, Shanghai, People’s Republic of ChinaDepartment of Obstetrics and Gynecology of Shanghai Medical School, Fudan University, Shanghai, People’s Republic of ChinaShanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, People’s Republic of ChinaDepartment of Obstetrics and Gynecology of Shanghai Medical School, Fudan University, Shanghai, People’s Republic of ChinaInstitute of Biomedical Sciences, Fudan University, Shanghai, People’s Republic of ChinaObstetrics and Gynecology Hospital of Fudan University, Shanghai, People’s Republic of ChinaDepartment of Obstetrics and Gynecology of Shanghai Medical School, Fudan University, Shanghai, People’s Republic of ChinaShanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, People’s Republic of ChinaShanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, People’s Republic of ChinaInstitute of Biomedical Sciences, Fudan University, Shanghai, People’s Republic of ChinaObstetrics and Gynecology Hospital of Fudan University, Shanghai, People’s Republic of ChinaDepartment of Obstetrics and Gynecology of Shanghai Medical School, Fudan University, Shanghai, People’s Republic of ChinaShanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, People’s Republic of China

关键词: Ovarian cancer stem cells;    Glycomic;    Biomarker;    Tn antigen;    T antigen;    sT antigen;   

DOI  :  10.1186/s12014-016-9131-z

来源: Humana Press Inc

【 摘 要 】

Abstract

Background

Ovarian cancer is the most lethal gynecological malignancy due to its frequent recurrence and drug resistance even after successful initial treatment. Accumulating scientific evidence indicates that subpopulations of cancer cells with stem cell-like properties, such as so-called side population (SP) cells, are primarily responsible for these recurrences. A better understanding of SP cells may provide new clues for detecting and targeting these cancer-initiating cells and ultimately help to eradicate cancer. Changes in glycosylation patterns are remarkable features of SP cells. Here, we isolated SP cells from ovarian cancer cell lines and analyzed their glycosylation patterns using multiple glycomic strategies.

Methods

Six high-grade serous ovarian cancer cell lines were used for SP cell isolation. Among them, HO8910 pm, which contained the highest proportion of SP cells, was used for glycomic analysis of SP cells. Cell lysate of SP cells and main population cells was applied to lectin microarray and mass spectrometry for glycan profiling. Differently expressed glycan structures were further verified by lectin blot, flow cytometry, and real-time PCR analysis of their relevant enzymes.

Results

Expression of core fucosylated N-glycan and tumor-associated Tn, T and sT antigens were increased in SP cells. By contrast, SP cells exhibited decreased hybrid glycan, α2,3-linked sialic glycan and multivalent sialyl-glycan.

Conclusions

Glycan structures, such as Tn, T, sT antigens, and core fucosylation may serve as biomarkers of ovarian cancer stem cells.

【 授权许可】

Unknown