Blood-sampling collection prior to surgery may have a significant influence upon biomarker concentrations measured

Clinical Proteomics

Sabine Schott-Hildebrand¹  Thomas R Muley⁴  Felix J Herth²  Marc A Schneider⁴  Michael Lindner¹  Julia Riedlinger³  Nicolas Kahn²  Christina Rabe³  Markus Roeßler³  Michael Meister⁴  Ina Koch¹ 

[1] Center of Thoracic Surgery, Asklepios Fachkliniken München-Gauting, Ludwig Maximilians University, Gauting, GermanyComprehensive Pneumology Centre Munich (CPC-M), German Centre for Lung Research (DZL), Munich, GermanyCenter of Thoracic Surgery, Asklepios Fachkliniken München-Gauting, Ludwig Maximilians University, Gauting, GermanyCenter of Thoracic Surgery, Asklepios Fachkliniken München-Gauting, Ludwig Maximilians University, Gauting, GermanyComprehensive Pneumology Centre Munich (CPC-M), German Centre for Lung Research (DZL), Munich, GermanyComprehensive Pneumology Centre Munich (CPC-M), German Centre for Lung Research (DZL), Munich, GermanyCenter of Thoracic Surgery, Asklepios Fachkliniken München-Gauting, Ludwig Maximilians University, Gauting, GermanyComprehensive Pneumology Centre Munich (CPC-M), German Centre for Lung Research (DZL), Munich, Germany;Department of Pneumology and Critical Care Medicine, Thoraxklinik, University of Heidelberg, Heidelberg, GermanyTranslational Lung Research Centre Heidelberg (TLRC-H), German Centre for Lung Research (DZL), Heidelberg, GermanyDepartment of Pneumology and Critical Care Medicine, Thoraxklinik, University of Heidelberg, Heidelberg, GermanyDepartment of Pneumology and Critical Care Medicine, Thoraxklinik, University of Heidelberg, Heidelberg, GermanyTranslational Lung Research Centre Heidelberg (TLRC-H), German Centre for Lung Research (DZL), Heidelberg, GermanyTranslational Lung Research Centre Heidelberg (TLRC-H), German Centre for Lung Research (DZL), Heidelberg, GermanyDepartment of Pneumology and Critical Care Medicine, Thoraxklinik, University of Heidelberg, Heidelberg, GermanyTranslational Lung Research Centre Heidelberg (TLRC-H), German Centre for Lung Research (DZL), Heidelberg, Germany;Roche Diagnostics GmbH, Mannheim, GermanyRoche Diagnostics GmbH, Mannheim, GermanyRoche Diagnostics GmbH, Mannheim, Germany;Translational Research Unit (STF), Thoraxklinik, University of Heidelberg, Heidelberg, GermanyTranslational Lung Research Centre Heidelberg (TLRC-H), German Centre for Lung Research (DZL), Heidelberg, GermanyTranslational Research Unit (STF), Thoraxklinik, University of Heidelberg, Heidelberg, GermanyTranslational Research Unit (STF), Thoraxklinik, University of Heidelberg, Heidelberg, GermanyTranslational Lung Research Centre Heidelberg (TLRC-H), German Centre for Lung Research (DZL), Heidelberg, GermanyTranslational Lung Research Centre Heidelberg (TLRC-H), German Centre for Lung Research (DZL), Heidelberg, GermanyTranslational Research Unit (STF), Thoraxklinik, University of Heidelberg, Heidelberg, GermanyTranslational Lung Research Centre Heidelberg (TLRC-H), German Centre for Lung Research (DZL), Heidelberg, Germany

关键词: Biomarker;    Blood specimens;    Sampling time points;    Lung cancer;    SCC;    CEA;    CYFRA 21-1;    Anesthesia induction;    SPREC coding;   

DOI  :  10.1186/s12014-015-9093-6

来源: Humana Press Inc

【 摘 要 】

Abstract

Background

Biomarkers can be subtle tools to aid the diagnosis, prognosis and monitoring of therapy and disease progression. The validation of biomarkers is a cumbersome process involving many steps. Serum samples from lung cancer patients were collected in the framework of a larger study for evaluation of biomarkers for early detection of lung cancer. The analysis of biomarker levels measured revealed a noticeable difference in certain biomarker values that exhibited a dependence of the time point and setting of the sampling. Biomarker concentrations differed significantly if taken before or after the induction of anesthesia and if sampled via venipuncture or arterial catheter.

Methods

To investigate this observation, blood samples from 13 patients were drawn 1–2 days prior to surgery (T1), on the same day by venipuncture (T2) and after induction of anesthesia via arterial catheter (T3). The biomarkers Squamous Cell Carcinoma antigen (CanAG SCC EIA, Fujirebio Diagnostics, Malvern, USA), Carcinoembrionic Antigen (CEA), and CYFRA 21-1 (Roche Diagnostics GmbH, Mannheim, Germany) were analyzed.

Results

SCC showed a very strong effect in relation to the sampling time and procedure. While the first two points in time (T1; T2) were highly comparable (median fold-change: 0.84; p = 0.7354; correlation ρ = 0.883), patients showed a significant increase (median fold-change: 4.96; p = 0.0017; correlation ρ = -0.036) in concentration when comparing T1 with the sample time subsequent to anesthesia induction (T3). A much weaker increase was found for CYFRA 21-1 at T3 (median fold-change: 1.40; p = 0.0479). The concentration of CEA showed a very small, but systematic decrease (median fold-change: 0.72; p = 0.0039).

Conclusions

In this study we show the unexpectedly marked influence of blood withdrawal timing (before vs. after anesthesia) and procedure (venous versus arterial vessel puncture) has on the concentration of the protein biomarker SCC and to a less extent upon CYFRA21-1. The potential causes for these effects remain to be elucidated in subsequent studies, however these findings highlight the importance of a standardized, controlled blood collection protocol for biomarker detection.

【 授权许可】

Unknown