Differential proteomic analysis of synovial fluid from rheumatoid arthritis and osteoarthritis patients

Clinical Proteomics

Harsha Gowda⁶  Akhilesh Pandey^1,13  Nandini A Sahasrabuddhe⁶  Mukesh Dhillon^1,11  Srinivas M Srikanth⁷  Ramesh Jois¹  Rajesh Raju⁶  Arivusudar Marimuthu⁶  Subramanian Shankar^1,11  Navjyot Kaur^1,11  Sujatha Mohan^1,12  Yashwanth Subbannayya⁴  Raja Sekhar Nirujogi⁷  Lavanya Balakrishnan⁵  Santosh Renuse⁸  YL Ramachandra⁹  TS Keshava Prasad^1,10  Mitali Bhattacharjee⁸  Sartaj Ahmad²  Vivek Vasudev³ 

[1] Department of Rheumatology, Fortis Hospitals, Bangalore, IndiaDepartment of Rheumatology, Fortis Hospitals, Bangalore, IndiaDepartment of Rheumatology, Fortis Hospitals, Bangalore, India;Institute of Bioinformatics, International Technology Park, Bangalore, IndiaManipal University, Manipal, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore, IndiaManipal University, Manipal, IndiaManipal University, Manipal, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore, IndiaManipal University, Manipal, India;Department of Rheumatology, Command Airforce Hospital, Bangalore, IndiaDepartment of Rheumatology, Command Airforce Hospital, Bangalore, IndiaDepartment of Rheumatology, Command Airforce Hospital, Bangalore, India;Institute of Bioinformatics, International Technology Park, Bangalore, IndiaRajiv Gandhi University of Health Sciences, Bangalore, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore, IndiaRajiv Gandhi University of Health Sciences, Bangalore, IndiaRajiv Gandhi University of Health Sciences, Bangalore, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore, IndiaRajiv Gandhi University of Health Sciences, Bangalore, India;Institute of Bioinformatics, International Technology Park, Bangalore, IndiaDepartment of Biotechnology, Kuvempu University, Shankaraghatta, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore, IndiaDepartment of Biotechnology, Kuvempu University, Shankaraghatta, IndiaDepartment of Biotechnology, Kuvempu University, Shankaraghatta, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore, IndiaDepartment of Biotechnology, Kuvempu University, Shankaraghatta, India;Institute of Bioinformatics, International Technology Park, Bangalore, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore, India;Institute of Bioinformatics, International Technology Park, Bangalore, IndiaCentre for Excellence in Bioinformatics, School of Life Sciences, Pondicherry University, Puducherry, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore, IndiaCentre for Excellence in Bioinformatics, School of Life Sciences, Pondicherry University, Puducherry, IndiaCentre for Excellence in Bioinformatics, School of Life Sciences, Pondicherry University, Puducherry, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore, IndiaCentre for Excellence in Bioinformatics, School of Life Sciences, Pondicherry University, Puducherry, India;Institute of Bioinformatics, International Technology Park, Bangalore, IndiaAmrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore, IndiaAmrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam, IndiaAmrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore, IndiaAmrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam, India;Department of Biotechnology, Kuvempu University, Shankaraghatta, IndiaDepartment of Biotechnology, Kuvempu University, Shankaraghatta, IndiaDepartment of Biotechnology, Kuvempu University, Shankaraghatta, India;Institute of Bioinformatics, International Technology Park, Bangalore, IndiaAmrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam, IndiaManipal University, Manipal, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore, IndiaAmrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam, IndiaManipal University, Manipal, IndiaAmrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore, IndiaAmrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam, IndiaManipal University, Manipal, IndiaManipal University, Manipal, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore, IndiaAmrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam, IndiaManipal University, Manipal, India;Department of Internal Medicine, Armed Forces Medical College, Pune, IndiaDepartment of Internal Medicine, Armed Forces Medical College, Pune, IndiaDepartment of Internal Medicine, Armed Forces Medical College, Pune, India;Laboratory for Integrated Bioinformatics, RIKEN Center for Integrative Medical Sciences (IMS-RCAI), Yokohama Institute, Kanagawa, JapanLaboratory for Integrated Bioinformatics, RIKEN Center for Integrative Medical Sciences (IMS-RCAI), Yokohama Institute, Kanagawa, JapanLaboratory for Integrated Bioinformatics, RIKEN Center for Integrative Medical Sciences (IMS-RCAI), Yokohama Institute, Kanagawa, Japan;McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, USADepartment of Oncology, Johns Hopkins University School of Medicine, Baltimore, USADepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, USADepartment of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, USAMcKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, USAMcKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, USADepartment of Oncology, Johns Hopkins University School of Medicine, Baltimore, USADepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, USADepartment of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, USADepartment of Oncology, Johns Hopkins University School of Medicine, Baltimore, USAMcKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, USADepartment of Oncology, Johns Hopkins University School of Medicine, Baltimore, USADepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, USADepartment of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, USADepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, USAMcKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, USADepartment of Oncology, Johns Hopkins University School of Medicine, Baltimore, USADepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, USADepartment of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, USADepartment of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, USAMcKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, USADepartment of Oncology, Johns Hopkins University School of Medicine, Baltimore, USADepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, USADepartment of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, USA

关键词: Arthritis;    Joint inflammation;    Cartilage degradation;    Extracellular matrix;   

DOI  :  10.1186/1559-0275-11-1

来源: Humana Press Inc

【 摘 要 】

Abstract

Background

Rheumatoid arthritis and osteoarthritis are two common musculoskeletal disorders that affect the joints. Despite high prevalence rates, etiological factors involved in these disorders remain largely unknown. Dissecting the molecular aspects of these disorders will significantly contribute to improving their diagnosis and clinical management. In order to identify proteins that are differentially expressed between these two conditions, a quantitative proteomic profiling of synovial fluid obtained from rheumatoid arthritis and osteoarthritis patients was carried out by using iTRAQ labeling followed by high resolution mass spectrometry analysis.

Results

We have identified 575 proteins out of which 135 proteins were found to be differentially expressed by ≥3-fold in the synovial fluid of rheumatoid arthritis and osteoarthritis patients. Proteins not previously reported to be associated with rheumatoid arthritis including, coronin-1A (CORO1A), fibrinogen like-2 (FGL2), and macrophage capping protein (CAPG) were found to be upregulated in rheumatoid arthritis. Proteins such as CD5 molecule-like protein (CD5L), soluble scavenger receptor cysteine-rich domain-containing protein (SSC5D), and TTK protein kinase (TTK) were found to be upregulated in the synovial fluid of osteoarthritis patients. We confirmed the upregulation of CAPG in rheumatoid arthritis synovial fluid by multiple reaction monitoring assay as well as by Western blot. Pathway analysis of differentially expressed proteins revealed a significant enrichment of genes involved in glycolytic pathway in rheumatoid arthritis.

Conclusions

We report here the largest identification of proteins from the synovial fluid of rheumatoid arthritis and osteoarthritis patients using a quantitative proteomics approach. The novel proteins identified from our study needs to be explored further for their role in the disease pathogenesis of rheumatoid arthritis and osteoarthritis. Sartaj Ahmad and Raja Sekhar Nirujogi contributed equally to this article.

【 授权许可】

Unknown