Study of Interaction of Native DNA with Iron(III)-(2,4-Dihydroxysalophen)chloride

Journal of the Korean Chemical Society

Mohammad Reza Azani¹  Azin Hassanpour¹  Abdol Khalegh Bordbar¹ 

关键词: Àڿܼ±-°¡½Ã±¤¼± ºÐ±¤¹ý;    DNA;    Schiff base;    Interaction;    Fluorescence;    UV-vis spectroscopy;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

In this study, iron(III)-2,4-dihydroxysalophen chloride (Fe(2,4-DHSalophen)Cl), has been synthesized by combination of 2,4-dihydroxysalophen (2,4-DHSalophen) with FeCl2 in a solvent system. This complex combination was characterized using UV-vis and IR spectroscopies. Subsequently, the interaction between native calf thymus deoxyribonucleic acid (ct-DNA) and Fe(2,4-DHSalophen)Cl, was investigated in 10 mM Tris/HCl buffer solution, pH 7.2, using UV-visible absorption and fluorescence spectroscopies, thermal denaturation technique and viscosity measurements. From spectrophotometric titration experiments, the binding constant of Fe(2,4-DHSalophen)Cl with ct-DNA was found to be (1.6 ¡¾ 0.2) ¡¿ 103 M-1. The fluorescence study represents the quenching effect of Fe(2,4-DHSalophen)Cl on bound ethidium bromide to DNA. The quenching process obeys linear Stern- Volmer equation in extended range of Fe(2,4-DHSalophen)Cl concentration. Thermal denaturation experiments represent the increasing melting temperature of DNA (about 4.3 oC) due to binding of Fe(2,4-DHSalophen)Cl. These results are consistent with a binding mode dominated by interactions with the groove of ct-DNA.

【 授权许可】

Unknown