The Development of Analysis System for Genes Related Disease Using Chemical Properties of DHPLC

Journal of the Korean Chemical Society

Youn-Hyoung Nam¹  Sang-Bum Park¹  Jong-Gyu Kim¹  Jae-Sik Lee¹  Won Kang¹ 

关键词: p53 Gene;    Breast Cancer;    PCR-DHPLC;    Mutation;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

p53 tumor-suppressor gene has emerged that it is related of breast cancer with high sensitivity. p53 gene is located on chromosome 17p13.1 in human with 20 kb. In this study we extracted DNA from 100 tissues of breast cancer patients and 103 normals. Then we confirmed single-nucleotide polymorphism(SNP) using PCR-DHPLC(polymerase chain reaction-denaturing high performance liquid chromatogrphy). Also, we studied SNP of samples using several columns to identify relation between packing materials of column and resolution. As a result, we identified 4 C/A, C/G genotypes(4%) in exon 5 and 37 T del genotypes(37%) in exon 8 among 100 breast cancer tissues and 2 in exon 5, 9 in exon 8 among 103 normal samples. In resolution test, we confirmed that PS-DVB(poly styrene-divinylbenzen) column is more efficient than C18 column.

【 授权许可】

Unknown