Research for Single Nucleotide Polymorphism of Midkine Gene in Korean Colorectal Cancer Patients

Journal of the Korean Chemical Society

Youn-Hyoung Nam¹  Sang-Bum Park¹  Sang-Man Han¹  Jae-Gu Choi¹  Won-Cheoul Jang¹ 

关键词: MK(midkine) Gene;    Colorectal Cancer;    RFLP-Gel Electrophoresis;    DHPLC;    Mutation;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Midkine (MK) is a heparin-binding growth factor specified by retinoic acid responsive gene. It plays an important role in development and carcinogenesis. The MK gene is located on chromosome 11q11.2 in human. A heterozygous G to transversion at the 62nd base in intron 3 of this gene has been in sporadic colorectal cancer. To clarify whether this polymorphism is associated with a cancer risk, a case-control study was conducted. We examined 75 colorectal cancer tissue specimens and 75 unaffected control specimens. Using RFLP and DHPLC, we identified that the G to T transversion was detected 21 in 75 (28%) colorectal cancer samples and 5 in 75 (6.7%) normal sample. Therefore, this genotype could represent a risk for colorectal cancer in Korean. RFLP-DHPLC analysis is more efficient than RFLP-gel electrophoresis analysis, because DHPLC is not so time-consuming, technically easy, highly sensitive, and has the advantage of automation.

【 授权许可】

Unknown