Association Between Type-specific HPV Infections and hTERT DNA Methylation in Patients with Invasive Cervical Cancer

Cancer Genomics - Proteomics

RENSKE D.M. STEENBERGEN⁵  LINA BUITRAGO⁴  SUZANNE M. GARLAND⁶  PABLO MORENO-ACOSTA³  MARCELA BURGOS²  OSCAR GAMBOA⁸  MÓNICA MOLANO⁷  JUAN CARLOS MEJÍA¹  RAYNER ALVAREZ³  SEPEHR N. TABRIZI⁶  NICOLÁS MORALES² 

[1] ncological Pathology Group, National Cancer Institute, Bogotá, Colombiancological Pathology Group, National Cancer Institute, Bogotá, Colombiancological Pathology Group, National Cancer Institute, Bogotá, Colombia;esearch Group in Cancer Biology, Research Branch, National Cancer Institute, Bogotá, Colombiaesearch Group in Cancer Biology, Research Branch, National Cancer Institute, Bogotá, Colombiaesearch Group in Cancer Biology, Research Branch, National Cancer Institute, Bogotá, Colombia;esearch Group in Cancer Biology, Research Branch, National Cancer Institute, Bogotá, Colombiaesearch Group in Radiobiology Clinical, Molecular and Cellular, National Cancer Institute, Bogotá, Colombiaesearch Group in Cancer Biology, Research Branch, National Cancer Institute, Bogotá, Colombiaesearch Group in Cancer Biology, Research Branch, National Cancer Institute, Bogotá, Colombiaesearch Group in Radiobiology Clinical, Molecular and Cellular, National Cancer Institute, Bogotá, Colombiaesearch Group in Radiobiology Clinical, Molecular and Cellular, National Cancer Institute, Bogotá, Colombiaesearch Group in Cancer Biology, Research Branch, National Cancer Institute, Bogotá, Colombiaesearch Group in Radiobiology Clinical, Molecular and Cellular, National Cancer Institute, Bogotá, Colombia;nit Group of Analysis, Research Branch, National Cancer Institute, Bogotá, Colombianit Group of Analysis, Research Branch, National Cancer Institute, Bogotá, Colombianit Group of Analysis, Research Branch, National Cancer Institute, Bogotá, Colombia;epartment of Pathology, VU University Medical Center, Amsterdam, the Netherlandsepartment of Pathology, VU University Medical Center, Amsterdam, the Netherlandsepartment of Pathology, VU University Medical Center, Amsterdam, the Netherlands;icrobiology and Infection Diseases, The Royal Women´s Hospital, Melbourne, VIC, Australiaurdoch Children's Research Institute, Parkville, VIC, Australiaepartment of Obstetrics and Gynaecology, University of Melbourne, Parkville, VIC, Australiaicrobiology and Infection Diseases, The Royal Women´s Hospital, Melbourne, VIC, Australiaicrobiology and Infection Diseases, The Royal Women´s Hospital, Melbourne, VIC, Australiaurdoch Children's Research Institute, Parkville, VIC, Australiaepartment of Obstetrics and Gynaecology, University of Melbourne, Parkville, VIC, Australiaurdoch Children's Research Institute, Parkville, VIC, Australiaicrobiology and Infection Diseases, The Royal Women´s Hospital, Melbourne, VIC, Australiaurdoch Children's Research Institute, Parkville, VIC, Australiaepartment of Obstetrics and Gynaecology, University of Melbourne, Parkville, VIC, Australiaepartment of Obstetrics and Gynaecology, University of Melbourne, Parkville, VIC, Australiaicrobiology and Infection Diseases, The Royal Women´s Hospital, Melbourne, VIC, Australiaurdoch Children's Research Institute, Parkville, VIC, Australiaepartment of Obstetrics and Gynaecology, University of Melbourne, Parkville, VIC, Australia;esearch Group in Cancer Biology, Research Branch, National Cancer Institute, Bogotá, Colombiaesearch Group in Radiobiology Clinical, Molecular and Cellular, National Cancer Institute, Bogotá, Colombiaicrobiology and Infection Diseases, The Royal Women´s Hospital, Melbourne, VIC, Australiaesearch Group in Cancer Biology, Research Branch, National Cancer Institute, Bogotá, Colombiaesearch Group in Cancer Biology, Research Branch, National Cancer Institute, Bogotá, Colombiaesearch Group in Radiobiology Clinical, Molecular and Cellular, National Cancer Institute, Bogotá, Colombiaicrobiology and Infection Diseases, The Royal Women´s Hospital, Melbourne, VIC, Australiaesearch Group in Radiobiology Clinical, Molecular and Cellular, National Cancer Institute, Bogotá, Colombiaesearch Group in Cancer Biology, Research Branch, National Cancer Institute, Bogotá, Colombiaesearch Group in Radiobiology Clinical, Molecular and Cellular, National Cancer Institute, Bogotá, Colombiaicrobiology and Infection Diseases, The Royal Women´s Hospital, Melbourne, VIC, Australiaicrobiology and Infection Diseases, The Royal Women´s Hospital, Melbourne, VIC, Australiaesearch Group in Cancer Biology, Research Branch, National Cancer Institute, Bogotá, Colombiaesearch Group in Radiobiology Clinical, Molecular and Cellular, National Cancer Institute, Bogotá, Colombiaicrobiology and Infection Diseases, The Royal Women´s Hospital, Melbourne, VIC, Australia;esearch Group in Radiobiology Clinical, Molecular and Cellular, National Cancer Institute, Bogotá, Colombianit Group of Analysis, Research Branch, National Cancer Institute, Bogotá, Colombiaesearch Group in Radiobiology Clinical, Molecular and Cellular, National Cancer Institute, Bogotá, Colombiaesearch Group in Radiobiology Clinical, Molecular and Cellular, National Cancer Institute, Bogotá, Colombianit Group of Analysis, Research Branch, National Cancer Institute, Bogotá, Colombianit Group of Analysis, Research Branch, National Cancer Institute, Bogotá, Colombiaesearch Group in Radiobiology Clinical, Molecular and Cellular, National Cancer Institute, Bogotá, Colombianit Group of Analysis, Research Branch, National Cancer Institute, Bogotá, Colombia

关键词: DNA methylation;    uterine cervical neoplasms;    telomerase reverse transcriptase;    papillomavirus infections;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

Background: There exists limited information on the role of hTERT methylation, and its association with type-specific HPV infections in cervical cancer. Materials and Methods: Eighty-seven frozen samples were analyzed for type-specific HPV infection using a GP5+/GP6+ PCR-RLB assay (RLB). hTERT DNA methylation analysis was performed using a newly developed PCR-RLB-hTERT. Results: Ninety-three percent of samples were HPV-positive and fifteen different types were detected. hTERT methylation analysis of region 1 revealed no methylation in 78.8% of the samples and partial methylation in 21.2%. In region two, 68.2% showed no methylation and 31.8% showed a pattern of partial methylation. An association between the alpha 9 and alpha 7 species with a pattern of no methylation of hTERT in the region 1 was established (p=0.02 and p=0.03, respectively). Conclusion: Differences in patterns of methylation of the hTERT core promoter [region 1 (nt -208 to -1) and region 2 (nt +1 to +104) relative to first ATG] are related to the HPV species present.

【 授权许可】

Unknown