SATB1 is Down-regulated in Clear Cell Renal Cell Carcinoma and Correlates with miR-21-5p Overexpression and Poor Prognosis

Cancer Genomics - Proteomics

ZBIGNIEW KMIEC¹  AGNIESZKA SLIWINSKA-JEWSIEWICKA⁴  JANUSZ GODLEWSKI⁴  PRZEMYSLAW KWIATKOWSKI⁴  ANNA E. KOWALCZYK⁴  JOLANTA KIEWISZ⁴  BARTLOMIEJ E. KRAZINSKI⁴  PIOTR DZIEGIEL²  JEDRZEJ GRZEGRZOLKA³ 

[1] epartment of Human Histology and Embryology, Faculty of Medical Sciences, University of Warmia and Mazury in Olsztyn, Olsztyn, Polandepartment of Histology, Medical University of Gdansk, Gdansk, Polandepartment of Human Histology and Embryology, Faculty of Medical Sciences, University of Warmia and Mazury in Olsztyn, Olsztyn, Polandepartment of Human Histology and Embryology, Faculty of Medical Sciences, University of Warmia and Mazury in Olsztyn, Olsztyn, Polandepartment of Histology, Medical University of Gdansk, Gdansk, Polandepartment of Histology, Medical University of Gdansk, Gdansk, Polandepartment of Human Histology and Embryology, Faculty of Medical Sciences, University of Warmia and Mazury in Olsztyn, Olsztyn, Polandepartment of Histology, Medical University of Gdansk, Gdansk, Poland;epartment of Histology and Embryology, Wroclaw Medical University, Wroclaw, Polandepartment of Physiotherapy, Wroclaw University School of Physical Education, Wroclaw, Polandepartment of Histology and Embryology, Wroclaw Medical University, Wroclaw, Polandepartment of Histology and Embryology, Wroclaw Medical University, Wroclaw, Polandepartment of Physiotherapy, Wroclaw University School of Physical Education, Wroclaw, Polandepartment of Physiotherapy, Wroclaw University School of Physical Education, Wroclaw, Polandepartment of Histology and Embryology, Wroclaw Medical University, Wroclaw, Polandepartment of Physiotherapy, Wroclaw University School of Physical Education, Wroclaw, Poland;epartment of Histology and Embryology, Wroclaw Medical University, Wroclaw, Polandepartment of Histology and Embryology, Wroclaw Medical University, Wroclaw, Polandepartment of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland;epartment of Human Histology and Embryology, Faculty of Medical Sciences, University of Warmia and Mazury in Olsztyn, Olsztyn, Polandepartment of Human Histology and Embryology, Faculty of Medical Sciences, University of Warmia and Mazury in Olsztyn, Olsztyn, Polandepartment of Human Histology and Embryology, Faculty of Medical Sciences, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland

关键词: SATB1 expression;    miR-21-5p;    clear cell renal cell carcinoma;    clinicopathological parameters;    survival;    qPCR;    IHC;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

Background: Altered expression of special AT-rich sequence binding protein 1 (SATB1) was reported in several types of human cancers. This study aimed to determine the expression levels of SATB1, as well as miR-21-5p -the post-transcriptional repressor of SATB1 expression- in clear cell renal cell carcinoma (ccRCC) and to investigate their association with the progression of ccRCC. Materials and Methods: Immunohistochemistry and quantitative polymerase chain reaction were used to assess the expression of SATB1 protein and mRNA as well as miR-21-5p in tumor and matched normal kidney tissues collected from 56 ccRCC patients. Results: Nuclear SATB1 immunoreactivity was elevated in ccRCC cells while its cytoplasmic expression was decreased. SATB1 mRNA level was down-regulated in ccRCC tissue and inversely correlated with the content of miR-21-5p. Down-regulation of SATB1 mRNA and up-regulation of miR-21-5p were associated with shorter patient survival. Conclusion: Decreased expression of SATB1 in ccRCC may result from over-expressed miR-21-5p. Our data suggest that SATB1 may have a potential value as a prognostic marker in ccRCC.

【 授权许可】

Unknown