Single Nucleotide Polymorphisms of Genes for EGF, TGF-β and TNF-α in Patients with Pancreatic Carcinoma

Cancer Genomics - Proteomics

Guoyang Wu³  Lei Zhang²  Michael Keese¹  Florian Herrle²  Marco Niedergethmann² 

[1] Department for Vascular and Endovascular Surgery, Johann Wolfgang Goethe University Hospital Frankfurt, Frankfurt, GermanyDepartment of Surgery, University Hospital Mannheim, University Heidelberg, Heidelberg, GermanyDepartment for Vascular and Endovascular Surgery, Johann Wolfgang Goethe University Hospital Frankfurt, Frankfurt, GermanyDepartment for Vascular and Endovascular Surgery, Johann Wolfgang Goethe University Hospital Frankfurt, Frankfurt, GermanyDepartment of Surgery, University Hospital Mannheim, University Heidelberg, Heidelberg, GermanyDepartment of Surgery, University Hospital Mannheim, University Heidelberg, Heidelberg, GermanyDepartment for Vascular and Endovascular Surgery, Johann Wolfgang Goethe University Hospital Frankfurt, Frankfurt, GermanyDepartment of Surgery, University Hospital Mannheim, University Heidelberg, Heidelberg, Germany;Department of Surgery, University Hospital Mannheim, University Heidelberg, Heidelberg, GermanyDepartment of Surgery, University Hospital Mannheim, University Heidelberg, Heidelberg, GermanyDepartment of Surgery, University Hospital Mannheim, University Heidelberg, Heidelberg, Germany;Department of General Surgery, Zhongshan Hospital, Xiamen University, Xiamen, P.R. ChinaDepartment of General Surgery, Zhongshan Hospital, Xiamen University, Xiamen, P.R. ChinaDepartment of General Surgery, Zhongshan Hospital, Xiamen University, Xiamen, P.R. China

关键词: Pancreatic cancer;    polymorphism;    growth factor;    survival;    complications;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

Aim: To show whether single nucleotide polymorphisms (SNPs) of Epidermal growth factor (EGF)-61*A/G, Transforming growth factor beta 1 (TGF-B1) - 509*T/C and Tumor necrosis factor-alpha (TNF-A) -308*A/G are associated with the survival rate after pancreatic cancer surgery and with the frequency of post-operative complications. Patients and Methods: EGF 61*A/G, TGF-B1-509*T/C and TNF-A-308*A/G genotypes were analyzed in patients who underwent pylorus-preserving pancreaticoduonectomy for pancreatic carcinoma and were determined by means of Polymerase Chain Reaction–Restriction Fragment Length Polymorphism (PCR-RFLP). The association of each genetic polymorphism with clinical and pathological data of the patients and early tumor recurrence were evaluated. Results: A significantly lower median survival duration was found in EGF 61*AA homozygotes, as compared to the AG heterozygous group. There was also a significantly lower median survival duration in the TNF-A-308* AA homozygote group as compared to the AG and GG groups. Survival duration in patients had no correlation with TGF-B1 -509*T/C polymorphism. There was a significantly lower median survival duration in the TNF-A -308* AA homozygous group, as compared to the AG and GG group in a Cox proportional hazard model. The frequency of the TGF-B1 T-allele was higher among patients with leakage of the pancreatic anastomosis. The frequency of the TGF-B1 TC genotype was significantly higher among patients who developed leakage of the biliodigestive anastomosis as compared with the TGF-B1 CC genotype. The frequency of TGF-B1 T-carriers (i.e. TT+TC) was significantly higher among patients with leakage of the biliodigestive anastomosis, as compared to these with the TGF-B1 CC genotype. In a Cox proportional hazard model, only wound infection had a significant correlation with long-term survival duration of patients with pancreatic cancer. Conclusion: There appears to be a significant correlation of the EGF-61* AA and of the TNF-A -308* AA polymorphism with lower survival duration in patients with resectable pancreatic carcinoma. The presence of wound infection was associated with poor prognosis. TGF-B1-509* T-carrying genotypes were more frequent in paitents with severe post-operative complications.

【 授权许可】

Unknown