Identification of Differentially Expressed Proteins from Primary versus Metastatic Pancreatic Cancer Cells Using Subcellular Proteomics

Cancer Genomics - Proteomics

David Sindram³  Jin-Gyun Lee⁵  Herbert L. Bonkovsky⁴  David K. Han¹  Sun-Il Hwang²  Mark W. Russo⁴  Kimberly Q. Mckinney⁵ 

[1] Department of Cell Biology and Center for Vascular Biology, School of Medicine, University of Connecticut, Farmington, CT, U.S.A.Department of Cell Biology and Center for Vascular Biology, School of Medicine, University of Connecticut, Farmington, CT, U.S.A.Department of Cell Biology and Center for Vascular Biology, School of Medicine, University of Connecticut, Farmington, CT, U.S.A.;Proteomics Laboratory for Clinical and Translational Research, Carolinas HealthCare System, Charlotte, NC, U.S.A.Liver-Biliary-Pancreatic Center, Carolinas HealthCare System, Charlotte, NC, U.S.A.Proteomics Laboratory for Clinical and Translational Research, Carolinas HealthCare System, Charlotte, NC, U.S.A.Proteomics Laboratory for Clinical and Translational Research, Carolinas HealthCare System, Charlotte, NC, U.S.A.Liver-Biliary-Pancreatic Center, Carolinas HealthCare System, Charlotte, NC, U.S.A.Liver-Biliary-Pancreatic Center, Carolinas HealthCare System, Charlotte, NC, U.S.A.Proteomics Laboratory for Clinical and Translational Research, Carolinas HealthCare System, Charlotte, NC, U.S.A.Liver-Biliary-Pancreatic Center, Carolinas HealthCare System, Charlotte, NC, U.S.A.;Liver-Biliary-Pancreatic Center, Carolinas HealthCare System, Charlotte, NC, U.S.A.Liver-Biliary-Pancreatic Center, Carolinas HealthCare System, Charlotte, NC, U.S.A.Liver-Biliary-Pancreatic Center, Carolinas HealthCare System, Charlotte, NC, U.S.A.;Liver-Biliary-Pancreatic Center, Carolinas HealthCare System, Charlotte, NC, U.S.A.Department of Medicine, Carolinas HealthCare System, Charlotte, NC, U.S.A.Liver-Biliary-Pancreatic Center, Carolinas HealthCare System, Charlotte, NC, U.S.A.Liver-Biliary-Pancreatic Center, Carolinas HealthCare System, Charlotte, NC, U.S.A.Department of Medicine, Carolinas HealthCare System, Charlotte, NC, U.S.A.Department of Medicine, Carolinas HealthCare System, Charlotte, NC, U.S.A.Liver-Biliary-Pancreatic Center, Carolinas HealthCare System, Charlotte, NC, U.S.A.Department of Medicine, Carolinas HealthCare System, Charlotte, NC, U.S.A.;Proteomics Laboratory for Clinical and Translational Research, Carolinas HealthCare System, Charlotte, NC, U.S.A.Proteomics Laboratory for Clinical and Translational Research, Carolinas HealthCare System, Charlotte, NC, U.S.A.Proteomics Laboratory for Clinical and Translational Research, Carolinas HealthCare System, Charlotte, NC, U.S.A.

关键词: Pancreatic cancer;    metastasis;    subcellular proteomics;    molecular signature;    BXPC-3;    Capan-2;    SU.86.86;    Capan-1 cells;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

Pancreatic cancer is an aggressive disease with nearly equal yearly rates of diagnosis and death. Current therapies have failed to improve outcomes due to rapid disease progression and late stage at presentation. Recently, pathways involved in progression and metastasis have been elucidated; however, new knowledge has not generated more effective therapies. We report on the use of subcellular fractionation and liquid chromatography (LC)-mass spectrometry to identify 3,907 proteins in four pancreatic cancer cell lines, 540 of which are unique to primary cancer cells, and 487 unique to cells derived from metastatic sites. Statistical analysis identified 134 proteins significantly differentially expressed between the two populations. The subcellular localization of these proteins was determined and expression levels for four targets were validated using western blot techniques. These identified proteins can be further investigated to determine their roles in progression and metastasis and may serve as therapeutic targets in the development of more effective treatments for pancreatic cancer.

【 授权许可】

Unknown