Modulation of Apoptosis Protein Profiles – Role of P-gp in HeLa Cells Exposed to Doxorubicin

Cancer Genomics - Proteomics

JOLANTA KRZYSZTOŃ – RUSSJAN²  BEATA M. GRUBER²  IRENA BUBKO²  ELŻBIETA L. ANUSZEWSKA²  MIROSŁAWA KORONKIEWICZ¹ 

[1] epartment of Cell Biology, National Medicines Institute, Warsaw, Polandepartment of Cell Biology, National Medicines Institute, Warsaw, Polandepartment of Cell Biology, National Medicines Institute, Warsaw, Poland;epartment of Biochemistry and Biopharmaceuticals, National Medicines Institute, Warsaw, Polandepartment of Biochemistry and Biopharmaceuticals, National Medicines Institute, Warsaw, Polandepartment of Biochemistry and Biopharmaceuticals, National Medicines Institute, Warsaw, Poland

关键词: Sulindac;    doxorubicin;    gene expression profile;    apoptosis;    P-glycoprotein;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

As shown previously doxorubicin (1 μM) plus sulindac (50 μM) reduced the expression of ABCB1 (ATP-binding cassette, sub-family B (MDR/TAP), member 1) mRNA in HeLa cells and this effect was accompanied by increased apoptosis. The aim of this study was to define if the decrease of ABCB1 expression or blocking of P-glycoprotein (P-gp) can affect the expression of the apoptotic genes determined with use of quantitative real time polymerase chain reaction (qRT-PCR). Western blot was used for visualization of chosen pro- and antiapoptotic proteins. Doxorubicin was the main compound which affected the apoptotic genes. The effectiveness of the drugs in reducing of P-gp function has been shown as not being related to the regulation of apoptotic gene transcription. In this experimental scheme, regulation of apoptotic gene transcription depended on the kind of P-gp modulator.

【 授权许可】

Unknown