Plasma Biomarkers Distinguish Non-small Cell Lung Cancer from Asthma and Differ in Men and Women

Cancer Genomics - Proteomics

David R. Campos³  Elzbieta Izbicka³  Robert T. Streeper³  Christopher L. Louden²  Joel E. Michalek²  o Diaz III³  Arm¹ 

[1]Department of Department of Epidemiology and Biostatistics, University of Texas Health Science Center at San Antonio, San Antonio, TX, U.S.A.Department of Department of Epidemiology and Biostatistics, University of Texas Health Science Center at San Antonio, San Antonio, TX, U.S.A.Department of Department of Epidemiology and Biostatistics, University of Texas Health Science Center at San Antonio, San Antonio, TX, U.S.A.

[2]Cancer Prevention and Cure Ltd, San Antonio, TX, U.S.A.Cancer Prevention and Cure Ltd, San Antonio, TX, U.S.A.Cancer Prevention and Cure Ltd, San Antonio, TX, U.S.A.

关键词: Biomarker;    non-small cell lung cancer;    asthma;    plasma;    multiplex assay;    autoantibodies;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

Background: Lung cancer (LC) is the leading cause of deaths caused by cancer worldwide. A diagnostic test for LC is needed for monitoring high-risk populations. Patients and Methods: Fifty-seven markers were measured using multiplex immunoassays of plasma of patients with non-small cell lung cancer (NSCLC); (245 men, 114 women, 1 unknown), asthma (67 men, 111 women, 2 unknown) and of healthy controls (165 men, 122 women, 1 unknown). Mass spectrometry was used for biomarker discovery. A support vector machine (SVM) was used for data analysis. Results: When all biomarkers and both genders were co-analyzed, SVM classified NSCLC and asthma with an accuracy of 0.94. Restricting to NSCLC versus healthy using best subsets of variables (males: epidermal growth factor (EGF), interleukin-8 (IL-8), soluble Fas (sFas), matrix metalloproteinase-9 (MMP-9), plasminogen activator inhibitor-1 (PAI-1); females: EGF, soluble cluster of differentiation 40 (sCD40) ligand, IL-8) yielded sensitivity and specificity of 1. Expression of eleven mass spectrometric biomarkers differed between pathologies. Conclusion: Significant inter-pathology and gender differences between biomarkers may improve diagnosis of LC.

【 授权许可】

Unknown