Proteomic Study of Sera from Patients with Bladder Cancer: Usefulness of S100A8 and S100A9 Proteins

Cancer Genomics - Proteomics

SHO MINAMI³  TAIHEI KAGEYAMA⁴  JUN-ICHIRO ISHII⁵  YUICHI SATO¹  TOSHIHIDE MATSUMOTO⁴  KODERA YOSHIO²  RYO NAGASHIO⁶  KAZUMASA MATSUMOTO⁵  ISAO OKAYASU⁴  YUSUKE KAWASHIMA² 

[1] Department of Molecular Diagnosis, Kitasato University Graduate School of Medical Sciences, Kanagawa, JapanDepartment of Cellular and Histo-Pathology, Kitasato University Graduate School of Medical Sciences, Kanagawa, JapanDepartment of Molecular Diagnostics, Kitasato University School of Allied Health Sciences, Kanagawa, JapanDepartment of Molecular Diagnosis, Kitasato University Graduate School of Medical Sciences, Kanagawa, JapanDepartment of Molecular Diagnosis, Kitasato University Graduate School of Medical Sciences, Kanagawa, JapanDepartment of Cellular and Histo-Pathology, Kitasato University Graduate School of Medical Sciences, Kanagawa, JapanDepartment of Molecular Diagnostics, Kitasato University School of Allied Health Sciences, Kanagawa, JapanDepartment of Cellular and Histo-Pathology, Kitasato University Graduate School of Medical Sciences, Kanagawa, JapanDepartment of Molecular Diagnosis, Kitasato University Graduate School of Medical Sciences, Kanagawa, JapanDepartment of Cellular and Histo-Pathology, Kitasato University Graduate School of Medical Sciences, Kanagawa, JapanDepartment of Molecular Diagnostics, Kitasato University School of Allied Health Sciences, Kanagawa, JapanDepartment of Molecular Diagnostics, Kitasato University School of Allied Health Sciences, Kanagawa, JapanDepartment of Molecular Diagnosis, Kitasato University Graduate School of Medical Sciences, Kanagawa, JapanDepartment of Cellular and Histo-Pathology, Kitasato University Graduate School of Medical Sciences, Kanagawa, JapanDepartment of Molecular Diagnostics, Kitasato University School of Allied Health Sciences, Kanagawa, Japan;Department of Physics, Kitasato University School of Science, Kanagawa, JapanDepartment of Physics, Kitasato University School of Science, Kanagawa, JapanDepartment of Physics, Kitasato University School of Science, Kanagawa, Japan;Department of Molecular Diagnosis, Kitasato University Graduate School of Medical Sciences, Kanagawa, JapanDepartment of Molecular Diagnosis, Kitasato University Graduate School of Medical Sciences, Kanagawa, JapanDepartment of Molecular Diagnosis, Kitasato University Graduate School of Medical Sciences, Kanagawa, Japan;Department of Cellular and Histo-Pathology, Kitasato University Graduate School of Medical Sciences, Kanagawa, JapanDepartment of Cellular and Histo-Pathology, Kitasato University Graduate School of Medical Sciences, Kanagawa, JapanDepartment of Cellular and Histo-Pathology, Kitasato University Graduate School of Medical Sciences, Kanagawa, Japan;Department of Urology, Kitasato University School of Science, Kanagawa, JapanDepartment of Urology, Kitasato University School of Science, Kanagawa, JapanDepartment of Urology, Kitasato University School of Science, Kanagawa, Japan;Department of Molecular Diagnostics, Kitasato University School of Allied Health Sciences, Kanagawa, JapanDepartment of Molecular Diagnostics, Kitasato University School of Allied Health Sciences, Kanagawa, JapanDepartment of Molecular Diagnostics, Kitasato University School of Allied Health Sciences, Kanagawa, Japan

关键词: S100A8;    S100A9;    bladder cancer;    serum;    proteomics;    immunohistochemistry;    clinical outcome;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

To effectively obtain tumour-specific markers, fractionated proteins obtained using reversed-phase high-performance liquid chromatography for patient-matched pre- and postoperative sera from bladder cancer patients were compared by two-dimensional gel electrophoresis. The usefulness of the identified proteins was confirmed immunohistochemically. S100A8 and S100A9 were identified as tumour-associated proteins. The increased immunoreactive expression of S100A8 protein was associated with bladder wall muscle invasion of the tumour and cancer-specific survival (p<0.05), and the increased immunoreactive expression of S100A9 protein was associated with the tumour grade (p<0.05). In addition, increased expressions of both proteins was associated with recurrence-free survival at a median follow-up of 32.9 months (both p<0.05). On multivariate analysis, the expression of S100A8 was a significant predictor of recurrence (p<0.05). These findings may help to identify biologically aggressive tumors and, thus, patients who might benefit from more intensive adjuvant therapy.

【 授权许可】

Unknown