Molecular Profiling Using Tissue Microarrays as a Tool to Identify Predictive Biomarkers in Laryngeal Cancer Treated with Radiotherapy

Cancer Genomics - Proteomics

KRISTINA MAGNUSSON³  MATTIAS UHLÉN⁴  PALLAVI JONNALAGADDA²  KARIN HELLSTRÖM⁹  MICHAEL BERGQVIST⁶  FREDRIK PONTÉN³  STEFAN BERGSTRÖM⁵  ANNA ASPLUND³  ERIK BLOMQUIST⁶  BRITTA LÖDÉN¹  SIMON EKMAN⁶  JOHAN REIZENSTEIN⁸  SARA STRÖMBERG³  MARTIN LILJEHOLM⁶  GEORG HOLGERSSON⁶  ARNE LINDER⁷ 

[1] epartment of Oncology, Central Hospital, SE-651 82 Karlstad, Swedenepartment of Oncology, Central Hospital, SE-651 82 Karlstad, Swedenepartment of Oncology, Central Hospital, SE-651 82 Karlstad, Sweden;ab Surgpath, 204 Bombay Market, Mumbai — 400034, Indiaab Surgpath, 204 Bombay Market, Mumbai — 400034, Indiaab Surgpath, 204 Bombay Market, Mumbai — 400034, India;epartment of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, SE-751 85 Uppsala, Swedenepartment of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, SE-751 85 Uppsala, Swedenepartment of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, SE-751 85 Uppsala, Sweden;TH - Royal Institute of Technology, AlbaNova University Center, Department of Proteomics, SE-106 91 Stockholm, SwedenTH - Royal Institute of Technology, AlbaNova University Center, Department of Proteomics, SE-106 91 Stockholm, SwedenTH - Royal Institute of Technology, AlbaNova University Center, Department of Proteomics, SE-106 91 Stockholm, Sweden;epartment of Oncology, Central Hospital, SE-801 87 Gävle, Swedenepartment of Oncology, Central Hospital, SE-801 87 Gävle, Swedenepartment of Oncology, Central Hospital, SE-801 87 Gävle, Sweden;epartment of Oncology, Uppsala University Hospital, SE-751 85 Uppsala, Swedenepartment of Oncology, Uppsala University Hospital, SE-751 85 Uppsala, Swedenepartment of Oncology, Uppsala University Hospital, SE-751 85 Uppsala, Sweden;epartment of Otorhinolaryngology and Head and Neck Surgery, Uppsala University Hospital, SE-751 85 Uppsala, Swedenepartment of Otorhinolaryngology and Head and Neck Surgery, Uppsala University Hospital, SE-751 85 Uppsala, Swedenepartment of Otorhinolaryngology and Head and Neck Surgery, Uppsala University Hospital, SE-751 85 Uppsala, Sweden;epartment of Oncology, Örebro University Hospital, SE-701 85 Örebro, Swedenepartment of Oncology, Örebro University Hospital, SE-701 85 Örebro, Swedenepartment of Oncology, Örebro University Hospital, SE-701 85 Örebro, Sweden;OC - Regional Oncologic Center in Uppsala/Örebro Region, SwedenOC - Regional Oncologic Center in Uppsala/Örebro Region, SwedenOC - Regional Oncologic Center in Uppsala/Örebro Region, Sweden

关键词: Laryngeal carcinoma;    Ki-67;    MSH2;    p53;    cyclin D1;    tissue microarray;    immunohistochemistry;    profiling;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

Aim: To explore the usefulness of the expression of five potential cancer biomarkers in predicting outcome in patients with laryngeal cancer. Materials and Methods: In the present study, the Swedish National Cancer Registry databases were used to identify patients with laryngeal cancer diagnosed during the years 1978-2004 in the Uppsala-Örebro region and treated with radiotherapy. The expression of Ki-67, MutS homolog 2, (MSH2), p53, B-cell CLL/lymphoma 2 (Bcl-2) and cyclin D1 in the cancer cells was assessed immunohistochemically using tissue microarrays (TMAs) and its predicitve value on survival and relapse was analyzed using Cox regression models. Results: A total of 39 patients were included in the present study. Nuclear MSH2 staining was statistically significantly correlated to Ki-67 expression (p=0.022). However, univariate and multivariate Cox analyses showed no statistically significant association between the expression of the investigated biomarkers and overall survival or relapse. Conclusion: The present exploratory study does not show any significant predictive value of the biomarkers examined with respect to survival or relapse. However, with larger patient cohorts, we believe that protein profiling using TMAs and immunohistochemistry is a feasible strategy for prognostic and predictive biomarker screening in laryngeal cancer.

【 授权许可】

Unknown