Immune Response Signatures of Benzo(α)pyrene Exposure in Normal Human Mammary Epithelial Cells in the Absence or Presence of Chlorophyllin

Cancer Genomics - Proteomics

AINSLEY WESTON³  JOGINDER NATH¹  DIANA L. RICHARDSON⁵  CHANNA KESHAVA²  KAARTHIK JOHN⁴ 

[1] enetics and Developmental Biology Program, West Virginia University, Morgantown, WVenetics and Developmental Biology Program, West Virginia University, Morgantown, WVenetics and Developmental Biology Program, West Virginia University, Morgantown, WV;oxicology and Molecular Biology Laboratory, National Institute for Occupational Safety and Health (NIOSH), Centers for Disease Control and Prevention (CDC), Morgantown, WVS Environmental Protection Agency, Research Triangle Park, NCoxicology and Molecular Biology Laboratory, National Institute for Occupational Safety and Health (NIOSH), Centers for Disease Control and Prevention (CDC), Morgantown, WVoxicology and Molecular Biology Laboratory, National Institute for Occupational Safety and Health (NIOSH), Centers for Disease Control and Prevention (CDC), Morgantown, WVS Environmental Protection Agency, Research Triangle Park, NCS Environmental Protection Agency, Research Triangle Park, NCoxicology and Molecular Biology Laboratory, National Institute for Occupational Safety and Health (NIOSH), Centers for Disease Control and Prevention (CDC), Morgantown, WVS Environmental Protection Agency, Research Triangle Park, NC;enetics and Developmental Biology Program, West Virginia University, Morgantown, WVoxicology and Molecular Biology Laboratory, National Institute for Occupational Safety and Health (NIOSH), Centers for Disease Control and Prevention (CDC), Morgantown, WVivision of Respiratory Disease Studies, NIOSH-CDC, Morgantown, WV, U.S.A.enetics and Developmental Biology Program, West Virginia University, Morgantown, WVenetics and Developmental Biology Program, West Virginia University, Morgantown, WVoxicology and Molecular Biology Laboratory, National Institute for Occupational Safety and Health (NIOSH), Centers for Disease Control and Prevention (CDC), Morgantown, WVivision of Respiratory Disease Studies, NIOSH-CDC, Morgantown, WV, U.S.A.oxicology and Molecular Biology Laboratory, National Institute for Occupational Safety and Health (NIOSH), Centers for Disease Control and Prevention (CDC), Morgantown, WVenetics and Developmental Biology Program, West Virginia University, Morgantown, WVoxicology and Molecular Biology Laboratory, National Institute for Occupational Safety and Health (NIOSH), Centers for Disease Control and Prevention (CDC), Morgantown, WVivision of Respiratory Disease Studies, NIOSH-CDC, Morgantown, WV, U.S.A.ivision of Respiratory Disease Studies, NIOSH-CDC, Morgantown, WV, U.S.A.enetics and Developmental Biology Program, West Virginia University, Morgantown, WVoxicology and Molecular Biology Laboratory, National Institute for Occupational Safety and Health (NIOSH), Centers for Disease Control and Prevention (CDC), Morgantown, WVivision of Respiratory Disease Studies, NIOSH-CDC, Morgantown, WV, U.S.A.;enetics and Developmental Biology Program, West Virginia University, Morgantown, WVoxicology and Molecular Biology Laboratory, National Institute for Occupational Safety and Health (NIOSH), Centers for Disease Control and Prevention (CDC), Morgantown, WVational Cancer Institute, Bethesda, MDenetics and Developmental Biology Program, West Virginia University, Morgantown, WVenetics and Developmental Biology Program, West Virginia University, Morgantown, WVoxicology and Molecular Biology Laboratory, National Institute for Occupational Safety and Health (NIOSH), Centers for Disease Control and Prevention (CDC), Morgantown, WVational Cancer Institute, Bethesda, MDoxicology and Molecular Biology Laboratory, National Institute for Occupational Safety and Health (NIOSH), Centers for Disease Control and Prevention (CDC), Morgantown, WVenetics and Developmental Biology Program, West Virginia University, Morgantown, WVoxicology and Molecular Biology Laboratory, National Institute for Occupational Safety and Health (NIOSH), Centers for Disease Control and Prevention (CDC), Morgantown, WVational Cancer Institute, Bethesda, MDational Cancer Institute, Bethesda, MDenetics and Developmental Biology Program, West Virginia University, Morgantown, WVoxicology and Molecular Biology Laboratory, National Institute for Occupational Safety and Health (NIOSH), Centers for Disease Control and Prevention (CDC), Morgantown, WVational Cancer Institute, Bethesda, MD;oxicology and Molecular Biology Laboratory, National Institute for Occupational Safety and Health (NIOSH), Centers for Disease Control and Prevention (CDC), Morgantown, WVoxicology and Molecular Biology Laboratory, National Institute for Occupational Safety and Health (NIOSH), Centers for Disease Control and Prevention (CDC), Morgantown, WVoxicology and Molecular Biology Laboratory, National Institute for Occupational Safety and Health (NIOSH), Centers for Disease Control and Prevention (CDC), Morgantown, WV

关键词: Carcinogens;    chemoprevention;    chlorophyllin;    gene expression;    polycyclic aromatic hydrocarbons;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

Carcinogenic polycylic aromatic hydrocarbons can alter immune responses. Changes in immune response gene expression profiles in multiple human mammary cell strains exposed to benzo(α)pyrene (BP) (4 μM) in vitro, in the presence or absence of chlorophyllin (5 μM), were observed using Affymetrix gene arrays. Expressions of five immune response genes were altered ~3.0-fold by BP exposure and 24 genes by BP in the presence chlorophyllin. In silico pathway analysis revealed altered immune response genes form interactive gene networks with many cellular processes, suggesting their role in a complex multigenic response to toxins. Additionally, it was suggestive of the possible immunomodulatory potential of chlorophyllin apart from various other well-documented mechanisms of action. Gene expression matrices revealed consistent alteration patterns involving IL1B, SECTM1 and CXCL14 on exposure to BP, and IL1RN, CD86, IF144 and GIP2 in the presence of chlorophyllin and BP, suggesting some of these genes might constitute putative immune response biomarkers of PAH exposure. This study has therefore identified a battery of potential immune response biomarkers of PAH exposure, amidst several genes, for future validation studies.

【 授权许可】

Unknown