Prognostic Utility of Glycosyltransferase Expression in Breast Cancer

Cancer Genomics - Proteomics

NEILL PATANI²  WEN JIANG¹  KEFAH MOKBEL³ 

[1] ardiff University, Cardiff, Walesardiff University, Cardiff, Walesardiff University, Cardiff, Wales;he London Breast Institute, The Princess Grace Hospital, Londonhe London Breast Institute, The Princess Grace Hospital, Londonhe London Breast Institute, The Princess Grace Hospital, London;he London Breast Institute, The Princess Grace Hospital, Londont. George's University of London, London, U.K.he London Breast Institute, The Princess Grace Hospital, Londonhe London Breast Institute, The Princess Grace Hospital, Londont. George's University of London, London, U.K.t. George's University of London, London, U.K.he London Breast Institute, The Princess Grace Hospital, Londont. George's University of London, London, U.K.

关键词: Breast cancer;    glycosylation;    prognosis;    survival;    recurrence;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

Background: The post-translational modification of proteins, including glycosylation, is known to differ between normal and tumour cells. In this study, the expression profile of two glycosyltranferases, UDP-N-acetyl-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase-6 (ppGalNAc-T6) and α6-sialyl-transferase-I (ST6GalNAc-I) was assessed, in a cohort of women with breast cancer. Patients and Methods: Breast cancer tissues (n=127) and normal background tissues (n=33) were collected immediately after excision during surgery. Following RNA extraction, reverse transcription was carried out and transcript levels were determined using real-time quantitative PCR and normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression. Transcript levels within the breast cancer specimens were compared to the normal background tissues and analyzed against conventional pathological parameters and clinical outcome over a 10 year follow-up period. Results: Significantly higher levels of ppGalNAc-T6 were found in the breast cancer specimens compared to the background tissue (p=0.015). There was a non-significant trend for levels to increase with the Nottingham Prognostic Index (NPI) and TNM stage and those who died from breast cancer. ST6GalNAc-I expression was associated with better prognosis, reaching significance when comparing patients who remained disease free to those with distant recurrence (p=0.0096). The relationship approached significance when comparing NPI 2 to NPI 3 (p=0.058) and disease free patients to non-disease free patients (p=0.052) or those who died of breast cancer (p=0.060). For both enzymes a significant association with ductal type was found. Conclusion: Expression of ppGalNAc-T6 is significantly higher in breast cancer compared to ‘normal’/benign breast tissue samples. ST6GalNAc-I expression in breast cancer is associated with better prognosis.

【 授权许可】

Unknown