Differential Expression of Ribosomal Proteins in a Human Metastasis Model Identified by Coupling 2-D Liquid Chromatography and Mass Spectrometry

Cancer Genomics - Proteomics

DAVID M. LUBMAN²  STEVE GOODISON¹  PAWEENA KREUNIN⁴  CHUL YOO⁴  VIRGINIA URQUIDI³ 

[1] epartment of Surgery, University of Florida, Jacksonville, FLepartment of Surgery, University of Florida, Jacksonville, FLepartment of Surgery, University of Florida, Jacksonville, FL;epartment of Chemistry, University of Michigan, Ann Arbor, MIepartment of Surgery, University of Michigan Medical School, Ann Arbor, MIomprehensive Cancer Center, University of Michigan Medical Center, Ann Arbor, MI, U.S.A.epartment of Chemistry, University of Michigan, Ann Arbor, MIepartment of Chemistry, University of Michigan, Ann Arbor, MIepartment of Surgery, University of Michigan Medical School, Ann Arbor, MIomprehensive Cancer Center, University of Michigan Medical Center, Ann Arbor, MI, U.S.A.epartment of Surgery, University of Michigan Medical School, Ann Arbor, MIepartment of Chemistry, University of Michigan, Ann Arbor, MIepartment of Surgery, University of Michigan Medical School, Ann Arbor, MIomprehensive Cancer Center, University of Michigan Medical Center, Ann Arbor, MI, U.S.A.omprehensive Cancer Center, University of Michigan Medical Center, Ann Arbor, MI, U.S.A.epartment of Chemistry, University of Michigan, Ann Arbor, MIepartment of Surgery, University of Michigan Medical School, Ann Arbor, MIomprehensive Cancer Center, University of Michigan Medical Center, Ann Arbor, MI, U.S.A.;epartment of Medicine, University of Florida, Jacksonville, FLepartment of Medicine, University of Florida, Jacksonville, FLepartment of Medicine, University of Florida, Jacksonville, FL;epartment of Chemistry, University of Michigan, Ann Arbor, MIepartment of Chemistry, University of Michigan, Ann Arbor, MIepartment of Chemistry, University of Michigan, Ann Arbor, MI

关键词: Ribosomal proteins;    cancer biomarkers;    electrospray ionization time-of-flight mass spectrometry;    liquid chromatography;    monolith;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

Background: Proteomic profiling of an experimental tumor metastasis model has the potential to identify gene products that can influence this fatal phenotype of tumor cells. In this study, we focused on the notoriously difficult to assess ribosomal protein component of a pair of cell lines which originate from the same tumor but have opposite metastatic capabilities. Materials and Methods: Cell lysate proteins were separated using a two-dimensional liquid chromatographic system directly coupled to an ESI-TOF mass spectrometer for accurate intact protein MW analysis. Characterization of distinct post-translational modifications and sequence variation within several ribosomal proteins was obtained using monolithic capillary LC/MS/MS, MALDI-MS and -MS/MS. Results: The combination of these techniques enabled the identification of 45 unique ribosomal proteins, several of which were differentially expressed in metastatic M4A4 cells. Conclusion: The described proteomic profiling approach enables the identification of phenotype-associated ribosomal proteins for subsequent functional analyses and disease biomarker development.

【 授权许可】

Unknown