A novel bacterial transport mechanism of Acinetobacter baumannii via activated human neutrophils through interleukin-8

Journal of Leukocyte Biology

Go Kamoshida³  Ryuichi Nakano¹  Satoshi Nishida³  Shouichi Higashi and²  Tsuneyuki Ubagai³  Shigeru Tansho-Nagakawa³  Yasuo Ono³  Takane Kikuchi-Ueda³  Kenji Hikosaka⁴ 

[1]Department of Microbiology and Immunology, Teikyo University School of Medicine, Tokyo, Japan

[2]  Department of Microbiology and Infectious Diseases, Nara Medical University, Nara, Japan

[3]Graduate School of Nanobioscience, Yokohama City University, Yokohama, Japan Department of Microbiology and Immunology, Teikyo University School of Medicine, Tokyo, Japan

[4]Department of Microbiology and Immunology, Teikyo University School of Medicine, Tokyo, Japan

[5]Department of Microbiology and Immunology, Teikyo University School of Medicine, Tokyo, Japan

[6] Department of Infection and Host Defense, Graduate School of Medicine, Chiba University, Chiba, Japan

关键词: adhesion;    in vitro transmigration assay;    invasion;    sepsis;   

DOI  :  10.1189/jlb.4AB0116-023RR

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Hospital-acquired infections as a result of Acinetobacter baumannii have become problematic because of high rates of drug resistance. Although neutrophils play a critical role in early protection against bacterial infection, their interactions with A. baumannii remain largely unknown. To elucidate the interactions between A. baumannii and human neutrophils, we cocultured these cells and analyzed them by microscopy and flow cytometry. We found that A. baumannii adhered to neutrophils. We next examined neutrophil and A. baumannii infiltration into Matrigel basement membranes by an in vitro transmigration assay. Neutrophils were activated by A. baumannii, and invasion was enhanced. More interestingly, A. baumannii was transported together by infiltrating neutrophils. Furthermore, we observed by live cell imaging that A. baumannii and neutrophils moved together. In addition, A. baumannii-activated neutrophils showed increased IL-8 production. The transport of A. baumannii was suppressed by inhibiting neutrophil infiltration by blocking the effect of IL-8. A. baumannii appears to use neutrophils for transport by activating these cells via IL-8. In this study, we revealed a novel bacterial transport mechanism that A. baumannii exploits human neutrophils by adhering to and inducing IL-8 release for bacterial portage. This mechanism might be a new treatment target.

【 授权许可】

Unknown