Splenic progenitors aid in maintaining high neutrophil numbers at sites of sterile chronic inflammation

Journal of Leukocyte Biology

Siddharth Jhunjhunwala⁵  David Alvarez¹  Peter E. Newburger,–³  Ulrich H. von Andrian,#¹  Katherine Tang⁵  Robert Langer, ,,  , and⁴  Benjamin C. Tang⁵  Stephanie Aresta-DaSilva⁵  Daniel G. Anderson, ,,  ,⁴  Dale L. Greiner² 

[1]Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, USA

[2] #The Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology, and Harvard, Cambridge, Massachusetts, USA

[3]Department of Molecular Medicine, Diabetes Center of Excellence, University of Massachusetts Medical School, Worcester, Massachusetts, USA

[4] #The Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology, and Harvard, Cambridge, Massachusetts, USA

[5]Department of Pediatrics, University of Massachusetts Medical School, Worcester, Massachusetts, USA

[6] –Department of Cancer Biology, University of Massachusetts Medical School, Worcester, Massachusetts, USA

[7] and #The Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology, and Harvard, Cambridge, Massachusetts, USA

[8]David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA

[9] Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA

[10]   Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA

[11] Harvard-Massachusetts Institute of Technology Division of Health Science and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA

[12]  Department of Anesthesiology, Boston Children™s Hospital, Boston, Massachusetts, USA

[13] Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, USA

[14] #The Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology, and Harvard, Cambridge, Massachusetts, USA

[15]David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA

[16]  Department of Anesthesiology, Boston Children™s Hospital, Boston, Massachusetts, USA

[17] #The Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology, and Harvard, Cambridge, Massachusetts, USA

关键词: granulocyte;    hematopoietic stem cells;    biomaterials;    devices;    peritoneal cavity;   

DOI  :  10.1189/jlb.1HI0615-248RR

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Neutrophils are constantly generated from hematopoietic stem and progenitor cells in the bone marrow to maintain high numbers in circulation. A considerable number of neutrophils and their progenitors have been shown to be present in the spleen too; however, their exact role in this organ remains unclear. Herein, we sought to study the function of splenic neutrophils and their progenitors using a mouse model for sterile, peritoneal inflammation. In this microcapsule device implantation model, we show chronic neutrophil presence at implant sites, with recruitment from circulation as the primary mechanism for their prevalence in the peritoneal exudate. Furthermore, we demonstrate that progenitor populations in the spleen play a key role in maintaining elevated neutrophil numbers. Our results provide new insight into the role for splenic neutrophils and their progenitors and establish a model to study neutrophil function during sterile inflammation.

【 授权许可】

Unknown