Microfluidic assay for precise measurements of mouse, rat, and human neutrophil chemotaxis in whole-blood droplets

Journal of Leukocyte Biology

Amy Mikkola³  Marina Yamada¹  Diane E. Brown , and⁵  Masao Kaneki¹  Caroline N. Jones²  H. Shaw Warren⁴  Yoshitaka Inoue²  Naohide Kuriyama¹  Laurie Dimisko²  Daniel Irimia,3²  Bashar Hamza²  Joseph M. Martel²  Anh N. Hoang² 

[1]Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA

[2]BioMEMS Resource Center, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA

[3] Center for Comparative Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA BioMEMS Resource Center, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA

[4]Department of Pediatrics and Medicine, Infectious Disease Unit, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA

[5]

[6]Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA

[7] and  Center for Comparative Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA

关键词: neutrophil;    migration;    inbred lines;   

DOI  :  10.1189/jlb.5TA0715-310RR

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Animal models of human disease differ in innate immune responses to stress, pathogens, or injury. Precise neutrophil phenotype measurements could facilitate interspecies comparisons. However, such phenotype comparisons could not be performed accurately with the use of current assays, as they require the separation of neutrophils from blood using species-specific protocols, and they introduce distinct artifacts. Here, we report a microfluidic technology that enables robust characterization of neutrophil migratory phenotypes in a manner independent of the donor species and performed directly in a droplet of whole blood. The assay relies on the particular ability of neutrophils to deform actively during chemotaxis through microscale channels that block the advance of other blood cells. Neutrophil migration is measured directly in blood, in the presence of other blood cells and serum factors. Our measurements reveal important differences among migration counts, velocity, and directionality among neutrophils from 2 common mouse strains, rats, and humans.

【 授权许可】

Unknown