Synergistic CD40 signaling on APCs and CD8 T cells drives efficient CD8 response and memory differentiation

Journal of Leukocyte Biology

Agnès Legrand and¹  Christiane Pontoux¹  Laëtitia Rapetti¹  Sylvain Meunier, ¹  Corinne Tanchot, ¹  Laurent Beziaud¹ 

[1] Institut National de la Santé et de la Recherche Médicale, INSERM U00, and  INSERM U970 Paris Cardiovascular Research Center, Université Paris Descartes, Paris, France

关键词: immune responses;    effector functions;    CD4;    CD40–CD40L pathway;    aCD40ab administration;   

DOI  :  10.1189/jlb.0611292

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

The role of CD4 help during CD8 response and memory differentiation has been clearly demonstrated in different experimental models. However, the exact mechanisms of CD4 help remain largely unknown and preclude replacement therapy to develop. Interestingly, studies have shown that administration of an agonist aCD40ab can substitute CD4 help in vitro and in vivo, whereas the targets of this antibody remain elusive. In this study, we address the exact role of CD40 expression on APCs and CD8 T cells using aCD40ab treatment in mice. We demonstrate that aCD40 antibodies have synergetic effects on APCs and CD8 T cells. Full efficiency of aCD40 treatment requires CD40 expression on both populations: if one of these cell populations is CD40-deficient, the CD8 T cell response is impaired. Most importantly, direct CD40 signaling on APCs and CD8 T cells affects CD8 T cell differentiation differently. In our model, CD40 expression on APCs plays an important but dispensable role on CD8 T cell expansion and effector functions during the early phase of the immune response. Conversely, CD40 on CD8 T cells is crucial and nonredundant for their progressive differentiation into memory cells. Altogether, these results highlight that CD40–CD40L-dependent and independent effects of CD4 help to drive a complete CD8 T cell differentiation.

【 授权许可】

Unknown