In vivo-transmigrated human neutrophils are resistant to antiapoptotic stimulation

Journal of Leukocyte Biology

Jennie Karlsson³  Karin Christenson³  Martina Sundqvist³  David P. Speert²  Claes Dahlgren and³  Charlotta Movitz¹  Lena Björkman³  Johan Bylund³ 

[1] Department of  Infectious Diseases, Section of Hematology, Sahlgrenska Academy at University of Gothenburg, Sweden;Department of Pediatrics and Centre for Understanding and Preventing Infections in Children, University of British Columbia, Vancouver, Canada Department of  Infectious Diseases, Section of Hematology, Sahlgrenska Academy at University of Gothenburg, Sweden;Department of Rheumatology and Inflammation Research and Department of  Infectious Diseases, Section of Hematology, Sahlgrenska Academy at University of Gothenburg, Sweden;

关键词: phagocyte;    granulocyte;    apoptosis;    transmigration;    skin chamber;    exudate;   

DOI  :  10.1189/jlb.0111051

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Neutrophils respond to microbial invasion or injury by transmigration from blood to tissue. Transmigration involves cellular activation and degranulation, resulting in altered levels of surface receptors and changed responsiveness to certain stimuli. Thus, fundamental functional changes are associated with neutrophil transmigration from blood to tissue. Neutrophils isolated from peripheral blood spontaneously enter apoptosis, a process that can be accelerated or delayed by different pro- or antiapoptotic factors. How tissue neutrophils that have transmigrated in vivo regulate cell death is poorly understood. In this study, in vivo-transmigrated neutrophils (tissue neutrophils) were collected using a skin chamber technique and compared with blood neutrophils from the same donors with respect to regulation of cell death. Skin chamber fluid contained a variety of cytokines known to activate neutrophils and regulate their lifespan. Freshly prepared tissue neutrophils had elevated activity of caspase 3/7 but were fully viable; spontaneous cell death after in vitro culture was also similar between blood and tissue neutrophils. Whereas apoptosis of cultured blood neutrophils was delayed by soluble antiapoptotic factors (e.g., TLR ligands), tissue neutrophils were completely resistant to antiapoptotic stimulation, even though receptors were present and functional. In vitro transmigration of blood neutrophils into skin chamber fluid did not fully confer resistance to antiapoptotic stimulation, indicating that a block of antiapoptotic signaling occurs specifically during in vivo transmigration. We describe a novel, functional alteration that takes place during in vivo transmigration and highlights the fact that life and death of neutrophils may be regulated differently in blood and tissue.

【 授权许可】

Unknown