The role of calcium signaling in phagocytosis

Journal of Leukocyte Biology

Nicolas Demaurex¹  Paula Nunes¹ 

[1] Department of Cell Physiology and Metabolism, University of Geneva, Geneva, SwitzerlandDepartment of Cell Physiology and Metabolism, University of Geneva, Geneva, SwitzerlandDepartment of Cell Physiology and Metabolism, University of Geneva, Geneva, Switzerland

关键词: ion channels;    neutrophils;    NADPH oxidase;   

DOI  :  10.1189/jlb.0110028

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Immune cells kill microbes by engulfing them in a membrane-enclosed compartment, the phagosome. Phagocytosis is initiated when foreign particles bind to receptors on the membrane of phagocytes. The best-studied phagocytic receptors, those for Igs (FcγR) and for complement proteins (CR), activate PLC and PLD, resulting in the intracellular production of the Ca2+-mobilizing second messengers InsP3 and S1P, respectively. The ensuing release of Ca2+ from the ER activates SOCE channels in the plasma and/or phagosomal membrane, leading to sustained or oscillatory elevations in cytosolic Ca2+ concentration. Cytosolic Ca2+ elevations are required for efficient ingestion of foreign particles by some, but not all, phagocytic receptors and stringently control the subsequent steps involved in the maturation of phagosomes. Ca2+ is required for the solubilization of the actin meshwork that surrounds nascent phagosomes, for the fusion of phagosomes with granules containing lytic enzymes, and for the assembly and activation of the superoxide-generating NADPH oxidase complex. Furthermore, Ca2+ entry only occurs at physiological voltages and therefore, requires the activity of proton channels that counteract the depolarizing action of the phagocytic oxidase. The molecules that mediate Ca2+ ion flux across the phagosomal membrane are still unknown but likely include the ubiquitous SOCE channels and possibly other types of Ca2+ channels such as LGCC and VGCC. Understanding the molecular basis of the Ca2+ signals that control phagocytosis might provide new, therapeutic tools against pathogens that subvert phagocytic killing.

【 授权许可】

Unknown