Induction of neutrophil degranulation by S100A9 via a MAPK-dependent mechanism

Journal of Leukocyte Biology

Denis Girard²  Jean-Christophe Simard²  Philippe A. Tessier¹ 

[1] Centre de Recherche en Infectiologie, Centre Hospitalier de l’Université Laval, and Faculty of Medicine, Laval University, Quebec, Canada Centre de Recherche en Infectiologie, Centre Hospitalier de l’Université Laval, and Faculty of Medicine, Laval University, Quebec, Canada Centre de Recherche en Infectiologie, Centre Hospitalier de l’Université Laval, and Faculty of Medicine, Laval University, Quebec, Canada;Laboratoire de Recherche en Inflammation et Physiologie des Granulocytes, Université du Québec, INRS-Institut Armand-Frappier, Laval, Quebec, Canada; and Laboratoire de Recherche en Inflammation et Physiologie des Granulocytes, Université du Québec, INRS-Institut Armand-Frappier, Laval, Quebec, Canada; and Laboratoire de Recherche en Inflammation et Physiologie des Granulocytes, Université du Québec, INRS-Institut Armand-Frappier, Laval, Quebec, Canada; and

关键词: inflammation;    granules;    flow cytometry;    zymography;   

DOI  :  10.1189/jlb.1009676

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

S100A9 is a proinflammatory protein, expressed abundantly in the cytosol of neutrophils and monocytes. High extracellular S100A9 concentrations have been correlated with chronic inflammatory diseases such as rheumatoid arthritis and Crohn’s disease, as well as with phagocyte extravasation. This study tested the hypothesis that S100A9 induces degranulation in human neutrophils. S100A9 was found to up-regulate the surface expression of CD35 and CD66b, proteins contained in secretory vesicles and specific/gelatinase granules, respectively. In addition, gelatinase and albumin, stored, respectively, in specific/gelatinase granules and secretory vesicles, were detected in the supernatants of neutrophils stimulated with S100A9. In contrast, stimulation with S100A9 had no effect on CD63 expression or MPO secretion, two proteins contained in azurophilic granules. S100A9 induced the phosphorylation of the MAPKs, ERK1/2, p38, and JNK. Inhibition of p38 and JNK but not ERK1/2, with specific inhibitors (SB203580, JNKII, and PD98059, respectively), blocked neutrophil degranulation induced by S100A9. Taken together, these results support the hypothesis and clearly indicate that S100A9 induces the degranulation of secretory and specific/gelatinase granules but not of azurophilic granules in a process involving p38 and JNK and further support its classification as a DAMP.

【 授权许可】

Unknown