Human monocyte-derived dendritic cells differentiated in the presence of IL-2 produce proinflammatory cytokines and prime Th1 immune response

Journal of Leukocyte Biology

Antonio Ciaramella¹  Nunzia Sanarico³  Silvia Vendetti⁶  Daniela Bernasconi²  Vittorio Colizzi⁴  Francesca Mariani⁸  Alessandra Sacchi⁵  Paola Bossù⁷ 

[1] Department of Biology, University of Rome Tor Vergata, Italy; Department of Clinical and Behavioural Neurology, IRCCS Santa Lucia Foundation, Rome, Italy; and Department of Biology, University of Rome Tor Vergata, Italy; Department of Biology, University of Rome Tor Vergata, Italy; Department of Clinical and Behavioural Neurology, IRCCS Santa Lucia Foundation, Rome, Italy; and Department of Clinical and Behavioural Neurology, IRCCS Santa Lucia Foundation, Rome, Italy; and Department of Biology, University of Rome Tor Vergata, Italy; Department of Clinical and Behavioural Neurology, IRCCS Santa Lucia Foundation, Rome, Italy; and;Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy; and Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy; and Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy; andDepartment of Biology, University of Rome Tor Vergata, Italy; Institute of Neurobiology and Molecular Medicine, Italian National Council of Research, Rome; Department of Biology, University of Rome Tor Vergata, Italy; Department of Biology, University of Rome Tor Vergata, Italy; Institute of Neurobiology and Molecular Medicine, Italian National Council of Research, Rome; Institute of Neurobiology and Molecular Medicine, Italian National Council of Research, Rome; Department of Biology, University of Rome Tor Vergata, Italy; Institute of Neurobiology and Molecular Medicine, Italian National Council of Research, Rome;;Department of Biology, University of Rome Tor Vergata, Italy; Centre International “Chantal Biya” Yaoundè, Cameroun Department of Biology, University of Rome Tor Vergata, Italy; Department of Biology, University of Rome Tor Vergata, Italy; Centre International “Chantal Biya” Yaoundè, Cameroun Centre International “Chantal Biya” Yaoundè, Cameroun Department of Biology, University of Rome Tor Vergata, Italy; Centre International “Chantal Biya” Yaoundè, CamerounNational Institute for Infectious Disease “L. Spallanzani,” IRCCS, Rome, Italy; National Institute for Infectious Disease “L. Spallanzani,” IRCCS, Rome, Italy; National Institute for Infectious Disease “L. Spallanzani,” IRCCS, Rome, Italy;;National Institute for Infectious Disease “L. Spallanzani,” IRCCS, Rome, Italy; Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy; and National Institute for Infectious Disease “L. Spallanzani,” IRCCS, Rome, Italy; National Institute for Infectious Disease “L. Spallanzani,” IRCCS, Rome, Italy; Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy; and Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy; and National Institute for Infectious Disease “L. Spallanzani,” IRCCS, Rome, Italy; Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy; and;Department of Clinical and Behavioural Neurology, IRCCS Santa Lucia Foundation, Rome, Italy; and Department of Clinical and Behavioural Neurology, IRCCS Santa Lucia Foundation, Rome, Italy; and Department of Clinical and Behavioural Neurology, IRCCS Santa Lucia Foundation, Rome, Italy; andInstitute of Neurobiology and Molecular Medicine, Italian National Council of Research, Rome; Institute of Neurobiology and Molecular Medicine, Italian National Council of Research, Rome; Institute of Neurobiology and Molecular Medicine, Italian National Council of Research, Rome;

关键词: antigen-presenting cell;    T cell polarization;    cell differentiation;   

DOI  :  10.1189/jlb.1105690

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Interleukin (IL)-2 plays an important role in the control of the immune responses, and it is released in a variety of tissues in response to inflammatory stimuli. As monocytes and mature dendritic cells (DCs) express CD25, the high-affinity subunit of IL-2 receptor, we examined the effect of exogenous IL-2 on the in vitro generation and maturation of DCs from monocytes. Human monocyte-derived DCs (MDDCs) were generated by culturing monocytes with granulocyte macrophage-colony stimulating factor (GM-CSF) and IL-4 in the presence or absence of IL-2. The cytokine was added at the beginning and after 5 days of culture. Our findings indicate that IL-2 does induce monocytes to differentiate into DCs with the same morphology and phenotype of that obtained in the presence of GM-CSF and IL-4 alone, but with some distinctive functional properties. DCs differentiated in the presence of IL-2 secreted significantly more IL-1β, TNF-α, and IL-12 p70 in response to lipopolysaccharide stimulation and induced allogeneic, naïve T cells to release a significantly higher amount of interferon-γ if compared with DCs obtained by culturing monocytes with GM-CSF and IL-4. These results indicate unrecognized effects of IL-2 on human MDDCs and suggest that an IL-2-rich environment during differentiation and maturation of DCs can modify their T helper cell-inducing properties.

【 授权许可】

Unknown