Detection and properties of the human proliferative monocyte subpopulation

Journal of Leukocyte Biology

Alice C. Holloway¹  Roya Lari²  Paul U. Cameron³  Felix I. L. Clanchy²  John A. Hamilton² 

[1] Departments of Medicine, Royal Melbourne Hospital, and Departments of Medicine, Royal Melbourne Hospital, and Departments of Medicine, Royal Melbourne Hospital, and;Departments of Medicine, Royal Melbourne Hospital, and Cooperative Research Centre for Chronic Inflammatory Diseases, Parkville, Victoria, Australia Departments of Medicine, Royal Melbourne Hospital, and Departments of Medicine, Royal Melbourne Hospital, and Cooperative Research Centre for Chronic Inflammatory Diseases, Parkville, Victoria, Australia Cooperative Research Centre for Chronic Inflammatory Diseases, Parkville, Victoria, Australia Departments of Medicine, Royal Melbourne Hospital, and Cooperative Research Centre for Chronic Inflammatory Diseases, Parkville, Victoria, Australia;Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia; and Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia; and Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia; and

关键词: macrophages;    cell proliferation;    cell differentiation;   

DOI  :  10.1189/jlb.0905522

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Peripheral blood monocyte subpopulations have been reported and can give rise to diverse, differentiated phenotypes. A subpopulation(s) of human monocytes can proliferate in vitro in response to macrophage-colony stimulating factor (M-CSF; or CSF-1). This population, termed the proliferative monocyte (PM), is presumably less mature than other monocytes; however, it has not been defined further. Previous studies monitoring the frequency of the slowly cycling PM from different donors indicated that the assay for their reproducible measurement required improvement. We demonstrate that for optimal PM detection, high 5-bromo-2′-deoxyuridine concentrations are required over a delayed and wide time-frame. Surface marker phenotyping by flow cytometry showed that freshly isolated PM are CD14+ and could be distinguished from two other human monocyte subpopulations, namely, the CD14loCD16+ and CD14loCD64– subsets. PM express relatively high levels of CD64 and CD33 but have relatively low CD13 expression; they are also c-Fms+ and human leukocyte antigen-DR+. Labeling with carboxyfluorescein diacetate succinimidyl ester (CFSE) enabled the estimation of the number of PM divisions over time. Following CFSE labeling and culture, PM were sorted from the nonproliferating population and shown to have a distinctive, spindle-shaped morphology and higher capacity to form multinucleated, tartrate-resistant acid phosphatase+ cells in the presence of M-CSF and receptor activator of nuclear factor-κB ligand. The phenotype and properties of the PM subpopulation were examined as a prelude to determining its role in disease using methods that can be applied to clarify human monocyte heterogeneity.

【 授权许可】

Unknown