Different signaling pathways inhibit DNA methylation activity and up-regulate IFN-γ in human lymphocytes

Journal of Leukocyte Biology

Francisco Sobrino¹  Raquel Martínez¹  Victoria Bonilla-Henao¹  Elizabeth Pintado¹ 

[1] Departamento de Bioquímica Médica y Biología Molecular, Facultad de Medicina y Hospital Universitario Virgen Macarena, Universidad de Sevilla, Spain Departamento de Bioquímica Médica y Biología Molecular, Facultad de Medicina y Hospital Universitario Virgen Macarena, Universidad de Sevilla, Spain Departamento de Bioquímica Médica y Biología Molecular, Facultad de Medicina y Hospital Universitario Virgen Macarena, Universidad de Sevilla, Spain

关键词: DNA methyltransferases;    PKC;    phosphatase inhibitors;    sulfhydryl-blocking reagents;   

DOI  :  10.1189/jlb.1004604

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

DNA methylation is recognized increasingly for its prominent role in controlling diverse immune processes. In this study, we show that in Jurkat T cells and fresh peripheral lymphocytes, short-time incubation with protein kinase C activators or phosphatase inhibitors down-regulate DNA methylation activity in a dose-dependent manner. This inhibition correlates with the induction of the interferon-γ (IFN-γ) gene, which contains several CG sequences in its promoter. The expression of mRNA and protein of the different DNA methyltransferases did not decrease after the treatment. In addition, sulfydryl reagents have a strong inhibitory effect on DNA methylation activity and also induce IFN-γ gene expression, thus suggesting a link between both effects.

【 授权许可】

Unknown