Analysis of the CCR7 expression on murine bone marrow-derived and spleen dendritic cells

Journal of Leukocyte Biology

Ziba Kiafard⁴  Heinrich Körner³  Uwe Ritter²  Florian Wiede²  Dirk Mielenz¹  Jörg Zwirner⁴ 

[1] Abteilung für Immunologie, Nikolaus-Fiebiger Zentrum für Molekulare Medizin, Universität Erlangen, Germany; Abteilung für Immunologie, Nikolaus-Fiebiger Zentrum für Molekulare Medizin, Universität Erlangen, Germany; Abteilung für Immunologie, Nikolaus-Fiebiger Zentrum für Molekulare Medizin, Universität Erlangen, Germany;;IZKF Nachwuchsgruppe 1, Nikolaus-Fiebiger Zentrum für Molekulare Medizin, and IZKF Nachwuchsgruppe 1, Nikolaus-Fiebiger Zentrum für Molekulare Medizin, and IZKF Nachwuchsgruppe 1, Nikolaus-Fiebiger Zentrum für Molekulare Medizin, and;IZKF Nachwuchsgruppe 1, Nikolaus-Fiebiger Zentrum für Molekulare Medizin, and Comparative Genomics Centre, James Cook University, Townsville, Australia IZKF Nachwuchsgruppe 1, Nikolaus-Fiebiger Zentrum für Molekulare Medizin, and IZKF Nachwuchsgruppe 1, Nikolaus-Fiebiger Zentrum für Molekulare Medizin, and Comparative Genomics Centre, James Cook University, Townsville, Australia Comparative Genomics Centre, James Cook University, Townsville, Australia IZKF Nachwuchsgruppe 1, Nikolaus-Fiebiger Zentrum für Molekulare Medizin, and Comparative Genomics Centre, James Cook University, Townsville, Australia;Abteilung für Immunologie, Georg-Augustus-Universität, Göttingen, Germany; and Abteilung für Immunologie, Georg-Augustus-Universität, Göttingen, Germany; and Abteilung für Immunologie, Georg-Augustus-Universität, Göttingen, Germany; and

关键词: tumor necrosis factor;    mouse;    knockout;    bone marrow precursor cells;    chemokine receptor;   

DOI  :  10.1189/jlb.0104037

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

About 40% of bone marrow-derived dendritic cells (BM-DCs) generated from stem cells of C57BL/6 (B6.WT) mice differentiate in the presence of granulocyte macrophage-colony stimulating factor (GM-CSF) without further stimuli to mature DCs. These cells are characterized by high levels of major histocompatibility complex class II, CD40, and CD86 on their surface. Recent studies have revealed that tumor necrosis factor (TNF) is crucial for maturation of BM-DCs. However, once matured, the phenotype of mature TNF-negative C57BL/6 (B6.TNF−/−) and B6.WT BM-DCs is comparable. Both expressed high levels of CD40 and CD86 and were positive for mRNA of the chemokine receptor (CCR)7. To extend our studies, we generated a monoclonal antibody (mAb) specific for mouse CCR7. This mAb allowed us to analyze the surface expression of CCR7 during maturation of B6.WT and B6.TNF−/− BM-DCs in the presence of GM-CSF and stimulated with TNF or lipopolysaccharide (LPS) and to compare it with the CCR7 expression on ex vivo-isolated splenic DCs with or without additional stimulation. Our results showed that CCR7 expression on murine BM-DCs is an indication of cell maturity. Incubation with LPS induced the maturation of all BM-DCs in culture but increased the number of mature CCR7+ splenic DCs only marginally.

【 授权许可】

Unknown