Lysophosphatidylcholine up-regulates CXCR4 chemokine receptor expression in human CD4 T cells

Journal of Leukocyte Biology

Ki Hoon Han¹  Myeong Ki Hong¹  Jae Joong Kim¹  Kyung Hee Hong¹  You Ho Kim¹  Kyong Suk Rhee¹  Seung Jung Park¹  Jesang Ko¹ 

[1] Asan Medical Center, University of Ulsan College of Medicine, Seoul, South KoreaAsan Medical Center, University of Ulsan College of Medicine, Seoul, South KoreaAsan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea

关键词: SDF-1;    atherosclerosis;    lysophosphatidyl-choline;    CXCR4;    CD4;    CD4 T cells;   

DOI  :  10.1189/jlb.1103563

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Oxidized low-density lipoprotein (OxLDL) is an inflammatory modulator in the atherosclerotic plaque. We examined the effect of lysophosphatidylcholine (lysoPC), a main phospholipid component of OxLDL, on inflammatory responses in human CD4 T cells. We found that lysoPC dose- and time-dependently increased expression of CXCR4, the chemokine receptor on CD4 T cells. This increase was inhibited by caffeic acid phenethyl ester or SN50, nuclear factor-κB inhibitors, and also by suppression of G2A expression, the specific receptor for lysoPC, using antisense oligonucleotide. lysoPC enhanced CD4 T cell chemotaxis in response to stromal cell-derived factor-1 (SDF-1), the exclusive ligand for CXCR4. lysoPC also enhanced SDF-1-stimulated production of inflammatory cytokines interleukin-2 and interferon-γ by CD4 T cells activated by anti-CD3 immunoglobulin G. In conclusion, this study demonstrates that lysoPC directly modulates inflammatory responses in human CD4 T cells. The data suggest that the presence of lysoPC and SDF-1 in atherosclerotic lesions may trigger inflammatory responses mediated by CD4 T cells, which may play an important role in progression of atherosclerosis.

【 授权许可】

Unknown