STI571 inhibits growth and adhesion of human mast cells in culture

Journal of Leukocyte Biology

Kenichi Koike²  Shuichi Ishida⁴  Masaaki Shiohara⁴  Kazuo Sakashita⁴  Tatsuya Kinoshita⁴  Kouichi Takeuchi⁴  Atsushi Komiyama⁴  Yozo Nakazawa⁴  Tetsuji Yamashita¹  Motowo Nakajima³  Yumi Kurokawa⁴  Shigeyuki Matsuzawa⁴  Takehiko Kamijo⁴ 

[1] Research & Development, Mitsubishi Kagaku Bio-Clinical Laboratories, Inc., Tokyo, Research & Development, Mitsubishi Kagaku Bio-Clinical Laboratories, Inc., Tokyo, Research & Development, Mitsubishi Kagaku Bio-Clinical Laboratories, Inc., Tokyo,;Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Shinshu University Graduate School of Medicine, Institute of Organ Transplants, Reconstructive Medicine and Tissue Engineering, Matsumoto, Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Shinshu University Graduate School of Medicine, Institute of Organ Transplants, Reconstructive Medicine and Tissue Engineering, Matsumoto, Shinshu University Graduate School of Medicine, Institute of Organ Transplants, Reconstructive Medicine and Tissue Engineering, Matsumoto, Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Shinshu University Graduate School of Medicine, Institute of Organ Transplants, Reconstructive Medicine and Tissue Engineering, Matsumoto,;Research Division, Tsukuba Research Institute, Novartis Pharma, Tsukuba, Japan. Research Division, Tsukuba Research Institute, Novartis Pharma, Tsukuba, Japan. Research Division, Tsukuba Research Institute, Novartis Pharma, Tsukuba, Japan.;Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Department of Pediatrics, Shinshu University School of Medicine, Matsumoto,

关键词: stem cell factor;    CD34⁺ cells;    c-kit;    cord blood;   

DOI  :  10.1189/jlb.0602284

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Stem cell factor (SCF)/c-kit system is critical for human mast cell development. We thus examined the effects of STI571, an inhibitor of the c-kit tyrosine kinase receptor, on the proliferation and function of human mast cells. STI571 at concentrations of 10−6 M or higher almost completely abolished the SCF-dependent progeny generation from cord blood-derived cultured mast cells through an inhibition of the tyrosine phosphorylation of c-kit. The compound also suppressed the early phase of mast cell development. The extinction of mast cell growth induced by STI571 may be due largely to apoptosis according to the flow cytometric analysis and gel electrophoresis. Two-hour exposure to STI571 that failed to influence the total viable cell number suppressed adhesion of the cells to fibronectin in the presence of SCF without altering the expressions of integrin molecules. Our results may provide a fundamental insight for the clinical application of STI571 in allergic disorders.

【 授权许可】

Unknown