Lactobacilli and streptococci induce inflammatory chemokine production in human macrophages that stimulates Th1 cell chemotaxis

Journal of Leukocyte Biology

Elena Giacomini²  Ville Veckman¹  Minja Miettinen¹  Roberto Lande²  Sampsa Matikainen¹  Ilkka Julkunen¹  Eliana M. Coccia² 

[1]Department of Microbiology, National Public Health Institute, Helsinki, Finland

[2] and Department of Microbiology, National Public Health Institute, Helsinki, Finland

[3] and Department of Microbiology, National Public Health Institute, Helsinki, Finland

[4] and

[5]Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy

关键词: bacteria;    chemokine;    cycloheximide;   

DOI  :  10.1189/jlb.0402212

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Macrophages have a central role in innate-immune responses to bacteria. In the present work, we show that infection of human macrophages with Gram-positive pathogenic Streptococcus pyogenes or nonpathogenic Lactobacillus rhamnosus GG enhances mRNA expression of inflammatory chemokine ligands CCL2/monocyte chemoattractant protein-1 (MCP-1), CCL3/macrophage-inflammatory protein-1α (MIP-1α), CCL5/regulated on activation, normal T expressed and secreted, CCL7/MCP-3, CCL19/MIP-3β, and CCL20/MIP-3α and CXC chemokine ligands CXCL8/interleukin (IL)-8, CXCL9/monokine induced by interferon-γ (IFN-γ), and CXCL10/IFN-inducible protein 10. Bacteria-induced CCL2, CCL7, CXCL9, and CXCL10 mRNA expression was partially dependent on ongoing protein synthesis. The expression of these chemokines and of CCL19 was dependent on bacteria-induced IFN-α/β production. CCL19 and CCL20 mRNA expression was up-regulated by IL-1β or tumor necrosis factor α (TNF-α), and in addition, IFN-α together with TNF-α further enhanced CCL19 gene expression. Synergy between IFN-α and TNF-α was also seen for CXCL9 and CXCL10 mRNA expression. Bacteria-stimulated macrophage supernatants induced the migration of T helper cell type 1 (Th1) cells, suggesting that in human macrophages, these bacteria can stimulate efficient inflammatory chemokine gene expression including those that recruit Th1 cells to the site of inflammation. Furthermore, L. rhamnosus-induced Th1 chemokine production could in part explain the proposed antiallergenic properties of this bacterium.

【 授权许可】

Unknown