Distinct populations of antigen-presenting macrophages are required for induction of effector and regulatory cells in contact sensitivity response in mice.

Journal of Leukocyte Biology

M Szczepanik¹  W Ptak¹  J Pryjma¹  K Bryniarski¹ 

[1] Department of Immunology, Institute of Pediatrics, Copernicus School of Medicine, Cracow, Poland.Department of Immunology, Institute of Pediatrics, Copernicus School of Medicine, Cracow, Poland.Department of Immunology, Institute of Pediatrics, Copernicus School of Medicine, Cracow, Poland.

DOI  :  

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Macrophages (Mf) and other antigen-presenting cells (APCs) are able to induce both immune and regulatory T cells. We compared the antigen-presenting activities of different subpopulations of thioglycolate-induced peritoneal macrophages from mice that were or were not treated with cyclophosphamide (CY) by several functional (adherence and phagocytosis) and morphologic (phenotypic) markers (FcR, Ia). Different subpopulations of macrophages were derivatized with trinitrophenyl and injected intravenously into recipients, which were tested directly for a contact sensitivity (CS) reaction or for the presence of efferent suppressor T (Ts) cells in passive transfer experiments. Our results demonstrate that peritoneal macrophages are both morphologically and functionally heterogeneous. Macrophages that induce immune cells that mediate CS have characteristics different from those that induce Ts cells. They accumulate in the low-density cell fraction on a discontinuous Ficoll gradient, are insensitive to treatment in vivo with low doses of CY, phagocytose poorly and adhere to plastic, and perhaps have low expression of FcRI and FcRII. Both macrophage fractions seem not to differ in expression of Ia, Mac-1, and Mac-3 antigens. It is argued that low doses of CY abrogate suppression in vivo by selective action on Ts cells. Our results confirm that at least a portion of the action of CY may be due to its influence on certain subpopulations of APCs.

【 授权许可】

Unknown