The Effects of Oltipraz on Tissue Regeneration in the Process of Wound Healing: A Stereological Study

Bulletin of Emergency And Trauma

Ali Noorafshan²  Mohammadreza Namazi³  Soheil Ashkani-Esfahani¹  Sina Kardeh⁴  Ehsan Saleh⁴ 

[1] Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran;Advanced trainee, Liverpool Hospital Dermatology Department, Liverpool, NSW, Sydney;Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran

关键词: Oltipraz;    Wound Healing;    Stereology;    Rat;   

DOI  :  

学科分类：基础医学

来源: Shiraz University of Medical Sciences

【 摘 要 】

Objective:To determine the effects of topical administration of 20% oltipraz solution on histomorphometrical and stereological aspects of skin tissue in full thickness skin wounds in laboratory rats. Methods:Thirty-six male Wistar portion rats (220±20 g) were randomly divided into three groups (n=12). On the first day of experimentation, a 1-cm 2circular wound was made on the posterior surface of neck in all rats by removing a full thickness skin piece immediately after induction of anesthesia with ether inhalation. One group was treated with vehicle solution (DMSO alone). The second group was treated daily with 20% oltipraz solution, and the third group, the control group, received no treatment. The wound closure rate was estimated our previously described method. The volume density of collagen bundles, vessels, and hair follicles, the vessels’ length density, mean diameter of vessels and also fibroblast population were estimated by using stereological methods. Results:The oltipraz group indicated a significantly higher improvement (6.26% of the wound surface per day) than control and the vehicle treated groups ( p =0.032); furthermore, there was inconsiderable difference between the rate of wound closure in the group treated with vehicle (4.93% per day) and the control group (4.43% per day). Conclusion:Oltipraz has positive influence on fibroblast proliferation and re-epithelization. A noticeable observation in our study was absence of scar formation in wounds which were treated by oltipraz and can be mentioned as an advantage of this drug.

【 授权许可】

Unknown