Quantification of sorgoleone in sorghum extracts and roots under different storage periods

Planta Daninha

Franco, F.H.S¹  Garcia, Q.S¹  Universidade Federal de Minas Gerais¹  UFMG, Belo Horizonte¹  Machado, Y¹  UFMG¹  Karam, D¹  Takahashi, J.A¹  Embrapa Milho e Sorgo, Sete Lagoas¹ 

关键词: Sorghum bicolor;    BR 007;    allelopathy;    purification;    standardization.;   

DOI  :  10.1590/S0100-83582011000500001

学科分类：农业科学（综合）

来源: Sociedade Brasileira da Ciencia das Plantas Daninhas (S B C P D) / Brazilian Society of Herb Science

【 摘 要 】

Sorgoleone (2 - hydroxy - 5 - methoxy - 3 - [(8'Z, 11'Z)-8',11',14'-pentadecatriene]-pbenzoquinone) was purified, identified by NMR, and quantified in primary roots of sorghum. The seeds were disinfected and stored in dark gerbox plates inside germination chambers at 30 ºC for seven days. Extraction was made by immersing the roots during 5 minutes in a glacial acetic acid/ dichloromethane solution 0, 0025% v/v. Sorgoleone quantification in the extracts was achieved by using a calibration curve made with the data obtained through High Performance Liquid Chromatography (HPLC). The effect of storing the extracts and roots was verified using three repetitions. The extracts of freshly collected roots were stored at -20ºC for 0, 1, 3, 5, 7, 14 and 21 days. No significant difference was observed between the amount of sorgoleone present in the stored and non-stored extracts. The same result was found for the extracts obtained from fresh and stored roots. The results obtained showed that storage up to 21 days does not alter the content of sorgoleone in the extracts.

【 授权许可】

Unknown