Eletrolite leakage as a technique to diagnose euphorbia heterophylla biotypes resistant to ppo-inhibitors herbicides

Planta Daninha

Xavier, E¹  Vidal, R.A¹  Dedordi, G.F¹  Kruse, N.D¹  Universidade Federal do Rio Grande do Sul, Porto Alegre¹  Trezzi, M.M¹  Gustman, M.S¹  Universidade Federal de Santa Maria, Santa Maria¹  Universidade Tecnológica Federal do Paraná, Pato Branco¹  Rosin, D¹ 

关键词: cell disruption;    electrical conductivity;    herbicide absorption;    resistance mechanism;    wild poinsettia.;   

DOI  :  10.1590/S0100-83582011000300020

学科分类：农业科学（综合）

来源: Sociedade Brasileira da Ciencia das Plantas Daninhas (S B C P D) / Brazilian Society of Herb Science

【 摘 要 】

The action of herbicides that affect the integrity of cell membranes and cause leakage, like PPO-inhibitors, can be detected by measuring the electric conductivity (EC) of a solution in which the plant tissue target is incubated in the presence of herbicide. The objectives of this work were to confirm PPO resistance in a new Euphorbia heterophylla (EPHHL) biotype, and to compare the electrolyte leakage from R and S to PPO-inhibitors biotypes, using two different methods of incubation in a solution containing herbicides. One experiment was carried in greenhouse and three in laboratory, with a completely randomized design. In the greenhouse experiment, four biotypes of EPHHL were sprayed with seven rates of fomesafen to confirm resistance in suspected biotypes. Leaf disks from R and S EPHHL biotypes in the second and the third experiments and entire leaves in the fourth experiment were incubated in a solution containing PPO-inhibitors to subsequently determine EC of solution. The study confirmed the resistance to PPO-inhibitors in two EPHHL biotypes. There were no significant differences between S and R biotypes in the experiments with the incubation of leaf disks, but incubation of entire leaves of EPHHL S biotype showed higher EC when in a solution with fomesafen, in comparison to the R biotype. The results of this work are an indirect evidence that resistance to PPO-inhibitors is related to lower absorption of herbicide by the shoots and also to some kind of mechanism to cope with oxidative stress.

【 授权许可】

Unknown