Evaluation and optimization of SYBR Green real-time reverse transcription polymerase chain reaction as a tool for diagnosis of the Flavivirus genus in Brazil

Revista da Sociedade Brasileira de Medicina Tropical

Universidade de São Paulo, Ribeirão Preto, Brazil¹  Figueiredo, Luiz Tadeu Moraes¹  Colombo, Tatiana Elias¹  Souza, William Marciel de¹  Tolardo, Aline Lavado¹  Romeiro, Marilia Farignoli¹  Vieira, Luiz Carlos¹  Aquino, Victor Hugo¹  Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, Brazil¹  Nogueira, Maurício Lacerda¹ 

关键词:  Flavivirus;    Arbovirus;    Real-time RT-PCR;    Diagnosis;   

DOI  :  10.1590/0037-8682-0444-2015

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

INTRODUCTION: The genus Flavivirus includes several pathogenic species that cause severe illness in humans. Therefore, a rapid and accurate molecular method for diagnosis and surveillance of these viruses would be of great importance. Here, we evaluate and optimize a quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) method for the diagnosis of the Flavivirus genus.

【 授权许可】

Unknown