Polymerase chain reaction-based method for the identification of Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in mucosal tissues conserved in paraffin

Revista da Sociedade Brasileira de Medicina Tropical

Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, Manaus, Brazil¹  Instituto de Higiene e Medicina Tropical, Lisboa, Portugal¹  Santana, Rosa Amelia Gonçalves¹  Guerra, Jorge Augusto de Oliveira¹  Universidade de Brasília, Brasília, Brazil¹  Silveira, Henrique¹  Universidade do Estado do Amazonas, Manaus, Brazil¹  Barbosa, Maria das Graças Vale¹  Custódio, Ana¹  Prestes, Suzane Ribeiro¹  Maciel, Marcel Gonçalves¹  Magalhaes, Laylah Kelre Costa¹  Romero, Gustavo Adolfo Sierra¹ 

关键词:  PCR;    Leishmania braziliensis;    Leishmania guyanensis;    Mucosal leishmaniasis;    Molecular diagnosis;   

DOI  :  10.1590/0037-8682-0132-2015

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

INTRODUCTION: In the Americas, mucosal leishmaniasis is primarily associated with infection by Leishmania (Viannia) braziliensis. However, Leishmania (Viannia) guyanensis is another important cause of this disease in the Brazilian Amazon. In this study, we aimed at detecting Leishmaniadeoxyribonucleic acid (DNA) within paraffin-embedded fragments of mucosal tissues, and characterizing the infecting parasite species.

【 授权许可】

Unknown