期刊论文详细信息
Revista da Sociedade Brasileira de Medicina Tropical
A comparison of four DNA extraction protocols for the analysis of urine from patients with visceral leishmaniasis
Melo, Fábio Lopes de1  Soares, Cynthia Regina Pedrosa1  Miranda-Filho, Demócrito Barros1  Medeiros, Zulma1  Silva, Elis Dionísio da1  Silva, Maria Almerice Lopes da1  Universidade de Pernambuco, Recife, Brasil1  Centro de Pesquisas Aggeu Magalhães, Recife, Brasil1 
关键词: Urine;    Extraction;    DNA;    Visceral leishmaniasis;   
DOI  :  10.1590/0037-8682-0233-2013
学科分类:农业科学(综合)
来源: Sociedade Brasileira de Medicina Tropical
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【 摘 要 】

IntroductionPolymerase chain reaction (PCR) may offer an alternative diagnostic option when clinical signs and symptoms suggest visceral leishmaniasis (VL) but microscopic scanning and serological tests provide negative results. PCR using urine is sensitive enough to diagnose human visceral leishmaniasis (VL). However, DNA quality is a crucial factor for successful amplification.

【 授权许可】

Unknown   

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