Natural infection with Leishmania infantum chagasi in Lutzomyia (Lutzomyia) longipalpis (Diptera: Psychodidae) sandflies captured in the municipality of Janaúba, State of Minas Gerais, Brazil

Revista da Sociedade Brasileira de Medicina Tropical

Universidade Federal de Minas Gerais, Belo Horizonte¹  Barata, Ricardo Andrade¹  Lara e Silva, Fabiana de Oliveira¹  Fundação Ezequiel Dias, Belo Horizonte¹  Michalsky, Érika Monteiro¹  França-Silva, João Carlos¹  Guedes, Karla de Sena¹  Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina¹  Dias, Consuelo Latorre Fortes¹  Dias, Edelberto Santos¹  Fundação Oswaldo Cruz, Belo Horizonte¹ 

关键词: Visceral leishmaniasis;    Natural infection;    Lutzomyia longipalpis;    Leishmania infantum chagasi;   

DOI  :  10.1590/S0037-86822011000100014

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

INTRODUCTION: Visceral leishmaniasis has been notified in nearly all states of Brazil, and particularly in the north of Minas Gerais, where the disease is endemic. The aim of this study was to detect natural infection of Lutzomyia longipalpis and, through the PCR/RFLP technique, identify Leishmania species found in sandflies in the municipality of Janaúba.METHODS: Using light traps, 1,550 females of L. longipalpis were caught and grouped into pools of 10 specimens to be subjected to DNA extraction and amplification, by means of generic PCR and cacophony.RESULTS: Out of the 155 pools, six were positive for Leishmania sp., and thus the infection rate in the municipality was 3.9%. Through PCR/RFLP, the digestion pattern among the positive samples was found to be similar to that of the reference strain of Leishmania chagasi (MHOM/BR/74/PP75).CONCLUSIONS: The detection of natural infection associated with studies on the epidemiology of visceral leishmaniasis suggests that L. longipalpis is involved in transmission of L. infantum chagasi in Janaúba, particularly in areas of intense transmission of visceral leishmaniasis.

【 授权许可】

Unknown