Oxacillin-resistant coagulase-negative Staphylococci bacteremia at a teaching hospital in Santa Maria, State of Rio Grande do Sul, Brazil

Revista da Sociedade Brasileira de Medicina Tropical

Martini, Rosiéli²  Mayer, Letícia Eichstaedt²  Domingues, Vanessa Oliveira²  França, Chirles Araújo de²  Rigatti, Fabiane¹  Tizotti, Maísa Kraulich¹  Khun, Fábio Teixeira²  Hörner, Rosmari¹  Costa, Mateus Matiuzzi da² 

[1] Universidade Federal de Santa Maria, Santa Maria

关键词: Bacteremia;    Nosocomial infection;    Coagulase-negative staphylococci;    mecA gene;    Cefoxitin;   

DOI  :  10.1590/S0037-86822010000600017

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

INTRODUCTION: This study aimed to characterize the prevalence and susceptibility profile to oxacillin-resistant Coagulase-negative Staphylococci strains isolated from blood cultures in a teaching hospital, located in Santa Maria, RS. In addition, different methodologies for phenotypic characterization of mecA-mediated oxacillin resistance were compared with genotypic reference testing.METHODS: After identification (MicroScan® - Siemens), the isolates were tested for antimicrobial sensitivity using disk diffusion and automation (MicroScan® - Siemens). The presence of mecA gene was identified by the polymerase chain reaction molecular technique.RESULTS: The most common species was Staphylococcus epidermidis (n=40, 67%). The mecA gene was detected in 54 (90%) strains, while analysis of the sensitivity profiles revealed a high rate of resistance to multiple classes of antimicrobial drugs. However, all isolates were uniformly sensitive to vancomycin and tigecycline. The cefoxitin disk was the phenotypic method that best correlated with the gold standard.CONCLUSIONS: Analysis of the clinical significance of CoNS isolated from hemocultures and the precise detection of oxacillin resistance represent decisive factors for the correct choice of antibiotic therapy. Although vancomycin constitutes the normal treatment in most Brazilian hospitals, reduction in its use is recommended.

【 授权许可】

Unknown