Bacterial contamination on platelet concentrates: identification, antimicrobial susceptibility profile and transfusion-related sepsis

Revista da Sociedade Brasileira de Medicina Tropical

Kuhn, Fábio Teixeira¹  Martini, Rosiéli¹  Ratzlaff, Viviane¹  Rodrigues, Mônica de Abreu¹  Segala, Zanoni¹  Hemocentro do Estado do Rio Grande do Sul, Santa Maria¹  Kempfer, Cláudia Barbisan¹  Rigatti, Fabiane¹  Universidade Federal de Santa Maria, Santa Maria¹  Hörner, Rosmari¹ 

关键词: Platelet Concentrate;    Bacterial contamination;    Sepsis;    Staphylococcus epidermidis;   

DOI  :  10.1590/S0037-86822010000600016

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

INTRODUCTION: Bacterial sepsis associated with the transfusion of platelet concentrates (PCs) results in serious clinical implications for patients. Given these implications, certain procedures have been integrated into the preparation and quality control of blood components to reduce the risk of bacterial contamination. This article describes the prevalence of bacterial contamination on transfused PCs, the bacterial spectrum detected and their antimicrobial susceptibility profile and transfusion reactions in receptors.METHODS: A total of 292 PCs (278 random and 14 per apheresis) from the Blood Center of the State of Rio Grande do Sul (HEMORGS), located in the city of Santa Maria, were tested. Quantities of 100μL and 200μL were collected from platelet bag tubing and seeded using two methodologies.RESULTS: Using the qualitative methodology, bacteria were isolated in five units (1.7%; 5/292), while only one was isolated using the quantitative methodology. Staphylococcus epidermidis was the microorganism identified in all samples. Two patients died of transfusion-related sepsis.CONCLUSIONS: Bacterial contamination due to PC transfusion is considered a major public health problem due to its association with high rates of morbidity and mortality. In this study only gram-positive microorganisms were isolated and none of the samples obtained by apheresis presented contamination.

【 授权许可】

Unknown