NRAMP1 gene polymorphisms in individuals with leprosy reactions attended at two reference centers in Recife, northeastern Brazil

Revista da Sociedade Brasileira de Medicina Tropical

Teixeira, Márcia Almeida Galvão¹  Universidade Federal de Pernambuco, Recife¹  Ramos, Alessandra de Luna¹  Silva, Norma Lucena¹  Universidade de Pernambuco, Recife¹  Instituto de Medicina Integrada Prof. Fernando Figueira de Pernambuco, Recife¹  Magalhães, Vera¹  Fundação Oswaldo Cruz, Rio de Janeiro¹  Hatagima, Ana¹ 

关键词: Leprosy;    Restriction fragment polymorphism;   

DOI  :  10.1590/S0037-86822010000300014

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

INTRODUCTION: To investigate susceptibility to leprosy reactions, three polymorphisms of the natural resistance-associated macrophage protein (NRAMP1) gene were determined in 201 individuals who were attended at two reference centers in Recife, between 2007 and 2008. Of these, 100 were paucibacillary and 101 were multibacillary.METHODS: The 274C/T, D543N and 1729+55del4 polymorphisms of the NRAMP1 gene were determined using the technique of restriction fragment polymorphism on DNA extracted from peripheral blood. Allelic and genotypic frequencies were estimated by direct counting.RESULTS: The predominant genotypes were: CC (51.8%) for 274C/T; GG (86.6%) for D543N; and +-TGTG (59.9%) for 1729+55del4. The mutant genotype 274 TT predominated in negativity of the reverse reaction (p = 0.03) and in positivity of erythema nodosum leprosum (p = 0.04).CONCLUSIONS: Our results suggest that 274 C/T polymorphism of the NRAMP1 gene may aid in determining the susceptibility to type II reactions among leprosy patients.

【 授权许可】

Unknown