Comparison of in vitro activity of five antifungal agents against dermatophytes, using the agar dilution and broth microdilution methods

Revista da Sociedade Brasileira de Medicina Tropical

Mota, Crystiane Rodrigues Araújo¹  Costa, Carolina Rodrigues¹  Lemos, Janine de Aquino¹  Silva, Maria do Rosário Rodrigues¹  Silva, Hildene Meneses e¹  Miranda, Karla Carvalho¹  Federal University of Goiás, Goiânia, Brazil¹  Souza, Lúcia Kioko Hasimoto e¹  Passos, Xisto Sena¹ 

关键词: In vitro susceptibility;    Agar dilution;    Broth microdilution;    Dermatophytes;   

DOI  :  10.1590/S0037-86822009000300003

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

The purpose of this study was to compare the agar dilution and broth microdilution methods for determining the minimum inhibitory concentration (MIC) of fluconazole, itraconazole, ketoconazole, griseofulvin and terbinafine for 60 dermatophyte samples belonging to the species Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum canis. The percentage agreement between the two methods, for all the isolates with < 2 dilutions that were tested was 91.6% for ketoconazole and griseofulvin, 88.3% for itraconazole, 81.6% for terbinafine and 73.3% for fluconazole. One hundred percent agreement was obtained for Trichophyton mentagrophytes isolates evaluated with ketoconazole and griseofulvin. Thus, until a reference method for testing the in vitro susceptibility of dermatophytes is standardized, the similarity of the results between the two methods means that the agar dilution method may be useful for susceptibility testing on these filamentous fungi.

【 授权许可】

Unknown