Serological reactivity of different antigenic preparations of Leishmania (Leishmania) amazonensis and the Leishmania braziliensis complex

Revista da Sociedade Brasileira de Medicina Tropical

Gomes-Silva, Adriano¹  Andrade, Lívia Resende¹  Dietze, Reynaldo¹  Souza, Maria Aparecida¹  Sustainable Sciences Institute, San Francisco, USA¹  Universidade Federal do Espírito Santo, Vitória¹  Ferreira, Marcelo Simão¹  Favoreto Júnior, Silvio¹  Universidade Federal de Uberlândia, Uberlândia¹  Belli, Alejandro¹  Lemos, Elenice¹  Afonso-Cardoso, Sandra Regina¹  Nothwestern University Feinberg School of Medicine, Chicago, USA¹ 

关键词: American tegmentary leishmaniasis;    Antigens from Leishmania braziliensis isolates;    Leishmania amazonensis antigens;    Concanavalin-A;    Jacalin;   

DOI  :  10.1590/S0037-86822008000200001

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

Total antigen from Leishmania (Leishmania) amazonensis and isolates from the Leishmania braziliensis complex, along with their respective antigenic fractions obtained by affinity chromatography on concanavalin-A-Sepharose and jacalin-agarose columns evaluated using immunoenzymatic ELISA assay. For this, serum samples from 229 patients were used, grouped as American tegmental leishmaniasis (nº=58), visceral leishmaniasis (nº=28), Chagas disease (nº=49), malaria (nº=32), tuberculosis (nº=13) and healthy volunteers (nº=49). Samples from American tegmentary leishmaniasis showed higher reactivity with antigens isolated from the Leishmania braziliensis complex than with antigens from Leishmania amazonensis (p<0.001). ELISA assays showed a sensitivity range from 60% to 95% with antigens isolated from the Leishmania braziliensis complex. There was marked nonspecific reactivity among serum samples with the use of antigenic fractions binding with concanavalin-A and jacalin from both Leishmania complexes, in comparison with other antigens (p<0.001). The results presented in this study suggest that the use of homologous antigens increases the efficiency of anti-Leishmania immunoglobulin detection, which may be very valuable for diagnostic purposes.

【 授权许可】

Unknown